Interaction of lipoprotein lipase and receptor-associated protein

The Journal of Biological Chemistry
Shallee PageA Bensadoun

Abstract

Receptor-associated protein (RAP) is a recognized chaperone/escort protein for members of the low density lipoprotein receptor family. In this report, we show that RAP binds to lipoprotein lipase (LPL) and may play a role in the maturation of LPL. Binding of highly purified RAP to LPL was demonstrated in vitro by solid phase assays, surface plasmon resonance, and rate zonal centrifugation. The dissociation constant for this interaction measured by the first two techniques ranged between 2.4 and 13 nM, values similar to those reported for the binding of RAP to LRP or gp330. The specificity of the interaction was demonstrated by competition with a panel of LPL monoclonal antibodies. Rate zonal centrifugation demonstrated the presence of a stable complex with an apparent Mr consistent with the formation of a complex between monomeric LPL and RAP. RAP x LPL complexes were co-immunoprecipitated in adipocyte lysates or from solutions of purified LPL and RAP. The interaction was also demonstrated in whole cells by cross-linking experiments. RAP-deficient adipocytes secreted LPL with a specific activity 2.5-fold lower than the lipase secreted by control cells. Heparin addition to cultured RAP-deficient adipocytes failed to stimulate LP...Continue Reading

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Citations

Oct 11, 2008·The Journal of Biological Chemistry·Michael M WeinsteinStephen G Young
Sep 26, 2012·Journal of Lipid Research·Brandon S J DaviesStephen G Young
May 12, 2012·Journal of Lipids·Ellen E QuillenMichael C Mahaney
Nov 6, 2007·The International Journal of Biochemistry & Cell Biology·Olga Ben-ZakenNeta Ilan
Nov 5, 2016·Journal of Lipid Research·Christopher M AllanStephen G Young
May 10, 2017·Journal of Lipid Research·Christopher M AllanStephen G Young
Jul 12, 2017·Journal of Lipid Research·Mikael LarssonLoren G Fong
Dec 13, 2007·Journal of Molecular Recognition : JMR·Rebecca L Rich, David G Myszka
Jan 2, 2019·Journal of Lipid Research·Christopher M AllanStephen G Young
Mar 26, 2009·American Journal of Physiology. Endocrinology and Metabolism·Hong Wang, Robert H Eckel
Nov 12, 2020·International Journal of Molecular Sciences·Kimberley D BruceRobert H Eckel

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