PMID: 3936544Nov 5, 1985Paper

Interaction of lysine residues with the metal thiolate clusters in metallothionein

Biochemistry
J PandeJ H Kägi

Abstract

Metallothioneins are unique diamagnetic metal thiolate cluster proteins. Both vertebrate and invertebrate forms contain, besides their large cysteine content (30%), up to 14% lysine plus arginine. In the amino acid sequences, the basic residues are juxtaposed to cysteine residues and have been suggested to play a role in neutralizing the excess negative charge of the metal thiolate complexes [Kojima, Y., Berger, C., Vallee, B. L., & Kägi, J. H. R. (1976) Proc. Natl. Acad. Sci. U.S.A. 73, 3413-3417]. To document such a function, we compared the susceptibility of the lysine residues in cadmium and zinc metallothioneins and in the metal-free S-carboxamidomethyl derivative toward arylation by trinitrobenzenesulfonic acid. The results show an at least 20-fold lower initial rate of reaction with the metal-containing as opposed to that with the metal-free form, indicating a protective effect of metal complex formation on the lysine residues, the degree of protection being dependent on the nature of the metal. The modification of the lysine residues by trinitrobenzenesulfonic acid produces changes in the CD spectral features of the cadmium thiolate cluster structure. The lowered chemical reactivity of the lysine residues in the metal-c...Continue Reading

References

Oct 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·Y KojimaJ H Kägi
Feb 15, 1974·FEBS Letters·R H Bühler, J H Kägi
Sep 1, 1971·The Biochemical Journal·R Fields
Oct 15, 1981·Biochemical and Biophysical Research Communications·E FinkelsteinE J Rauckman
Nov 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·M Vasák, J H Kägi
Mar 1, 1984·Environmental Health Perspectives·J H KägiM Good
Aug 1, 1984·The Biochemical Journal·D M Templeton, M G Cherian
Mar 1, 1984·Environmental Health Perspectives·D R Winge, K B Nielson
Mar 1, 1984·Environmental Health Perspectives·C T HuntI M Armitage
Dec 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·J D Otvos, I M Armitage
Dec 1, 1957·The Biochemical Journal·J M BAILEY, W J WHELAN

❮ Previous
Next ❯

Citations

Jun 10, 2004·Pathology Oncology Research : POR·Rongxian JinBoon-Huat Bay
Jul 1, 1989·Biological Trace Element Research·J H Kägi, P Hunziker
Dec 1, 1995·Environmental Health Perspectives·G MaroniL Theodore
Aug 5, 1998·Proceedings of the National Academy of Sciences of the United States of America·L J JiangB L Vallee
Feb 1, 1997·Drug Metabolism Reviews·P Moffatt, F Denizeau
Apr 27, 2011·Journal of Cellular Physiology·Debashree DeDebasish Bhattacharyya
Jul 1, 1988·Analytical Biochemistry·B A BrownS B Haber
Nov 15, 1988·Biochemistry·J H Kägi, A Schäffer
Jun 10, 2017·International Journal of Molecular Sciences·Artur Krężel, Wolfgang Maret
Apr 8, 2000·Critical Reviews in Biochemistry and Molecular Biology·A T MilesV Rodilla

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.