PMID: 9443072Jan 27, 1998Paper

Interaction of melittin with a human lymphoblastoid cell line, HMy2

Journal of Cellular Biochemistry
K M Weston, Robert L Raison

Abstract

We have examined the cytolytic effects of the membrane-active peptide, melittin, on a human lymphoblastoid cell line (HMy2) in the context of the use of melittin as the toxic component of an immunotoxin. The toxicity of melittin for HMy2 cells was linear over the concentration range 0.875-3.5 microM. Increased incubation times failed to result in significant cell death at concentrations of melittin below 0.875 microM. Kinetic analysis revealed that the cytolytic activity of melittin was independent of time of exposure beyond 90 min. Flow cytometric analysis of HMy2 cells incubated with FITC-labeled melittin demonstrated that the cells could incorporate up to 2.5 x 10(5) FITC-melittin molecules per cell with no reduction in viability. Extrapolation of this data indicates that 10(6) melittin molecules per cell are required for maximum cytotoxicity to HMy2 cells. Further analysis of HMy2 cells that incorporated melittin, but that remained viable, revealed that these cells were able to reduce the number of melittin molecules per cell over time. The data indicate a potential threshold value for the number of melittin molecules that may be required to be delivered to the cell surface in the form of an immunotoxin if effective selecti...Continue Reading

Citations

Feb 2, 2006·In Vitro Cellular & Developmental Biology. Animal·Juan Pablo PrattSteven J Mentzer
May 4, 2001·Journal of Immunological Methods·M SuS J Mentzer
Dec 26, 2015·Toxicon : Official Journal of the International Society on Toxinology·Goran GajskiVera Garaj-Vrhovac
Sep 1, 2015·Journal of the Chinese Medical Association : JCMA·Amir MahmoodzadehDelavar Shahbazzadeh
Dec 11, 2007·Biochemical Pharmacology·Sam Maher, Siobhán McClean
Jul 31, 2013·Environmental Toxicology and Pharmacology·Goran Gajski, Vera Garaj-Vrhovac

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