Interaction of Moloney murine leukemia virus matrix protein with IQGAP.

The EMBO Journal
Juliana LeungStephen P Goff

Abstract

The matrix protein (MA) of the Moloney murine leukemia virus (M-MuLV) was found to interact with IQGAP1, a prominent regulator of the cytoskeleton. Mutational studies defined residues of MA critical for the interaction, and tests of viruses carrying MA mutations revealed a near-perfect correlation between binding and virus replication. The replication-defective mutants showed defects in both early and late stages of the life cycle. Four viable second-site revertant viruses were isolated from three different replication-defective parental mutants, and in all cases the interaction with IQGAP1 was restored by the suppressor mutations. The interaction of MA and IQGAP1 was readily detected in vitro and in vivo. Virus replication was potently inhibited by a C-terminal fragment of IQGAP1, and impaired by RNAi knockdown of IQGAP1 and 2. We suggest that the IQGAPs link the virus to the cytoskeleton for trafficking both into and out of the cell.

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Citations

Feb 28, 2007·Nature Reviews. Microbiology·Stephen P Goff
May 3, 2013·Journal of Virology·Jianhong LuRonald N Harty
Jun 25, 2010·Journal of Virology·Matthew S HenningMojgan H Naghavi
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Dec 7, 2017·Frontiers in Cell and Developmental Biology·Andrea MolinasElena Vikström
May 20, 2016·Journal of Virology·Roger Valle-TenneyGloria Arriagada
Apr 19, 2019·Journal of Virology·Jenna Barnes, Duncan W Wilson
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Jul 1, 2008·Molecular and Cellular Biochemistry·Sevvel PathmanathanDavid J Timson

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