Oct 1, 1978

Interaction of mucopolysaccharides with glycosaminoglycans on glycosaminoglycan-bound AH-Sepharose 4B

Journal of Biochemistry
N Ototani, Z Yosizawa

Abstract

Chondroitinase C, chondroitinase AC, heparinase, and heparitinase separated from an extract of Flavobacterium heparinum were subjected to affinity chromatography with glycosaminoglycan-bound AH-Sepharose 4B, previously coated non-covalently with glycosaminoglycan, as the matrix. The results suggested the importance of coating the matrix with glycosaminoglycan in the binding of the enzyme protein to the matrix.

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Mentioned in this Paper

Glycosaminoglycans
Chondroitinases
Extracellular Matrix
Liquaemin
Heparitinase
Heparitin Sulfate
Polysaccharide-Lyases
AH-Sepharose 4B
Heparin Lyase
Sepharose

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