PMID: 8594541Oct 1, 1995Paper

Interaction of Na+ and Mg2+ ions in acetylcholine receptor channels of frog skeletal muscle changes in character with an increase in agonist concentration

Pflügers Archiv : European journal of physiology
A A Manthey

Abstract

Measurements of agonist-induced single-channel currents of nicotinic acetylcholine receptor channels (nAChR) have shown that addition of divalent metal cations (Ca2+, Mg2+, Ba2+) in millimolar amounts causes a decrease of 40% or more in currents carried by monovalent cations (Na+, Cs+). Conventional voltage-clamp measurements were made at frog (Rana pipiens) sartorius endplates to determine if this occlusive interaction between mono- and divalent cations is preserved in macroscopic currents produced by higher concentrations of nAChR agonists, as would be expected on the basis of simple summation of unitary currents. Single ion nAChR currents of Na+ (INa+ ) and Mg2+(IMg2+ ) and the composite current when both cations were present (INa+, Mg2+ ) were measured in high-K+, low ionic strength bathing media during local superfusion with various concentrations of carbamylcholine. It was found that, at lower test carbamylcholine levels of 27 and 54 microM, peak values of INa+, Mg2+ were 40% and 53% of the respective INa+ , in agreement with the effects observed from single-channel measurements. At all higher carbamylcholine levels, however, peak INa+, Mg2+ exceeded INa+ and increased instead with the sum of INa+ and IMg2+ as if there wa...Continue Reading

References

Jul 1, 1976·The Journal of Physiology·E Neher, B Sakmann
Nov 1, 1985·The American Journal of Physiology·A A Manthey
Jul 1, 1972·The International Journal of Neuroscience·J NegreteG Yankelevich
Aug 1, 1972·The Journal of Physiology·B Katz, R Miledi
Jan 1, 1982·Progress in Biophysics and Molecular Biology·J R BlinksF G Prendergast
May 1, 1982·The American Journal of Physiology·A A Manthey

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