PMID: 9555900Apr 29, 1998Paper

Interaction of native and mutant MecI repressors with sequences that regulate mecA, the gene encoding penicillin binding protein 2a in methicillin-resistant staphylococci

Journal of Bacteriology
V K SharmaGordon L Archer

Abstract

Methicillin resistance in staphylococci is mediated by PBP2a, a penicillin binding protein with low affinity for beta-lactam antibiotics. The gene encoding PBP2a, mecA, is transcriptionally regulated in some clinical isolates by mecR1 and mecI, genes divergently transcribed from mecA that encode a signal transducer and repressor, respectively. The biochemical basis of MecI-mediated mecA transcriptional repression was investigated by using purified MecI. In DNase I protection studies, MecI protected a 30-bp palindrome encompassing the predicted mecA -10 and the mecR1 -35 promoter sequences. The larger palindrome contained 15 bp of dyad symmetry within which was a smaller 6-bp palindrome. Electrophoretic mobility shift assays established a requirement for the entire 15-bp half-site for initial repressor binding. Fragments containing the 30-bp palindrome and the entire mecA-mecR1 intergenic region were retarded in gels as multiple discrete bands varying in molecular size, characteristic of cooperative DNA binding. Glutaraldehyde cross-linking confirmed oligomerization of repressor in solution. A naturally occurring MecI mutant (MecI*; D39G) repressed mecA transcription sixfold less well than the wild type in vivo. Although MecI* p...Continue Reading

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Citations

Sep 2, 2003·Journal of Bacteriology·Yuki KatayamaHenry F Chambers
Feb 28, 2006·Biometals : an International Journal on the Role of Metal Ions in Biology, Biochemistry, and Medicine·Reto PortmannMarc Solioz
Aug 17, 2006·FEMS Microbiology Reviews·Pauline MacheboeufAndréa Dessen
Jan 22, 2009·Molecular Microbiology·Claudia SalaStewart T Cole
Sep 24, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Marc SoliozStefano Mancini
Feb 13, 2004·The Journal of Biological Chemistry·Raquel García-CastellanosF Xavier Gomis-Rüth
Dec 26, 2001·Antimicrobial Agents and Chemotherapy·J E FinanGordon L Archer
Oct 8, 2008·The Biochemical Journal·Francesca CantiniMarc Solioz
Jun 5, 2016·Asian Pacific Journal of Tropical Medicine·Ryong KongDong-Yeul Kwon
Mar 26, 2003·Antimicrobial Agents and Chemotherapy·Adriana E RosatoGordon L Archer
Apr 24, 2002·Science Progress·Paul D Stapleton, Peter W Taylor
May 20, 2003·Journal of Bacteriology·Adriana E RosatoGordon L Archer
Jul 26, 2003·The Journal of Biological Chemistry·Raquel García-CastellanosF Xavier Gomis-Ruth
Mar 6, 2004·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Yuki KatayamaHenry F Chambers
Jun 4, 2015·Annual Review of Biochemistry·Sharon J Peacock, Gavin K Paterson
Jan 29, 2009·BMC Microbiology·Miriam EnderNadine McCallum
Mar 14, 2001·Science·G L Archer, J M Bosilevac
Dec 18, 2021·The Journal of Antimicrobial Chemotherapy·Ying JianMin Li

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