Interaction of NE-dlg/SAP102, a neuronal and endocrine tissue-specific membrane-associated guanylate kinase protein, with calmodulin and PSD-95/SAP90. A possible regulatory role in molecular clustering at synaptic sites

The Journal of Biological Chemistry
N MasukoH Saya

Abstract

NE-dlg/SAP102, a neuronal and endocrine tissue-specific membrane-associated guanylate kinase family protein, is known to bind to C-terminal ends of N-methyl-D-aspartate receptor 2B (NR2B) through its PDZ (PSD-95/Dlg/ZO-1) domains. NE-dlg/SAP102 and NR2B colocalize at synaptic sites in cultured rat hippocampal neurons, and their expressions increase in parallel with the onset of synaptogenesis. We have identified that NE-dlg/SAP102 interacts with calmodulin in a Ca2+-dependent manner. The binding site for calmodulin has been determined to lie at the putative basic alpha-helix region located around the src homology 3 (SH3) domain of NE-dlg/SAP102. Using a surface plasmon resonance measurement system, we detected specific binding of recombinant NE-dlg/SAP102 to the immobilized calmodulin with a Kd value of 44 nM. However, the binding of Ca2+/calmodulin to NE-dlg/SAP102 did not modulate the interaction between PDZ domains of NE-dlg/SAP102 and the C-terminal end of rat NR2B. We have also identified that the region near the calmodulin binding site of NE-dlg/SAP102 interacts with the GUK-like domain of PSD-95/SAP90 by two-hybrid screening. Pull down assay revealed that NE-dlg/SAP102 can interact with PSD-95/SAP90 in the presence of bo...Continue Reading

References

Dec 13, 2000·Journal of Molecular Recognition : JMR·R L Rich, D G Myszka
Feb 21, 2003·Current Opinion in Neurobiology·Aaron W McGee, David S Bredt
Jan 21, 2009·Nature Reviews. Neuroscience·Wei Feng, Mingjie Zhang
Jan 16, 2007·Nature Structural & Molecular Biology·Michael L ReeseVolker Dötsch
Mar 8, 2005·Nature Structural & Molecular Biology·Janet M MaltezGeoffrey S Pitt
Jun 26, 2002·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kazuyoshi KohuTetsu Akiyama
Oct 13, 2011·Proceedings of the National Academy of Sciences of the United States of America·Christopher A JohnstonK E Prehoda
Sep 28, 2005·The Journal of General Physiology·Shoji X TakahashiHenry M Colecraft
Nov 4, 2000·The EMBO Journal·H WuC C Garner
Jun 15, 2005·Annual Review of Biochemistry·Lars FunkeDavid S Bredt
Jun 15, 2006·Annual Review of Cell and Developmental Biology·Kunyoo ShinBen Margolis
May 1, 2012·PloS One·Christopher A JohnstonK E Prehoda
Mar 9, 2007·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Peter C CuthbertSeth G N Grant
Jan 15, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jason A BartosJohannes W Hell
Aug 16, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Stéphanie A D BonnetOliver M Schlüter
Jan 21, 2014·Biochemical and Biophysical Research Communications·Francisco J MuñozNibaldo C Inestrosa
Nov 9, 2000·Proceedings of the National Academy of Sciences of the United States of America·C RamponY Hu
Feb 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·C H JiangY Hu
Mar 14, 2007·The Journal of Cell Biology·Junichi IkenouchiShoichiro Tsukita
May 31, 2001·The Journal of Cell Biology·S TomitaDavid S Bredt
May 16, 2007·Progress in Histochemistry and Cytochemistry·L González-MariscalErika Garay
Jun 23, 2009·Annals of the New York Academy of Sciences·A S Fanning, J M Anderson
Sep 24, 2008·Protein Science : a Publication of the Protein Society·Ingo PaarmannManfred Konrad
Sep 23, 2006·The EMBO Journal·Lorraine V KaliaMichael W Salter
Aug 17, 1999·Current Opinion in Cell Biology·A S Fanning, J M Anderson
Nov 5, 2011·Current Opinion in Neurobiology·Patricio OpazoDaniel Choquet
Sep 1, 2000·Seminars in Cell & Developmental Biology·L González-MariscalA Avila-Flores
Oct 5, 2017·Proceedings of the National Academy of Sciences of the United States of America·Qi MaWei-Dong Yao
May 12, 2007·Journal of Neuroscience Research·Shintaro BesshohJames W Gurd
Aug 11, 2005·Journal of Biochemistry·Yuko FukunagaAtsuo Miyazawa
Nov 10, 2017·The EMBO Journal·Dhrubajyoti ChowdhuryJohannes W Hell

Citations

Jan 1, 1991·Methods in Enzymology·D M Becker, L Guarente
May 2, 1985·Nature·Y S BabuW J Cook
Dec 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M B KennedyN E Erondu
Jan 29, 1982·Biochemical and Biophysical Research Communications·R Gopalakrishna, W B Anderson
Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·K AlexandropoulosD Baltimore
Apr 25, 1994·Nucleic Acids Research·H E FeilotterD Beach
Aug 15, 1996·Nature·J H Morais CabralR C Liddington
May 2, 1997·The Journal of Biological Chemistry·M TakeuchiY Takai
Apr 1, 1997·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A R Rhoads, F Friedberg
Jun 1, 1997·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·K SatohT Akiyama
May 6, 1998·Current Opinion in Neurobiology·M Constantine-Paton, H T Cline

Related Concepts

Dlg3
Molecular Helix
Carboxy-Terminal Amino Acid
Presynaptic density protein 95
TJP1 gene
Helix (Snails)
GRIN2B wt Allele
Calmodulin
Calmodulin 1
Endocrine Tissue

Related Feeds

Brain developing: Influences & Outcomes

This feed focuses on influences that affect the developing brain including genetics, fetal development, prenatal care, and gene-environment interactions. Here is the latest research in this field.

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.