Interaction of nucleic acids with lipid membranes

Journal of Biomolecular Structure & Dynamics
B BrosiusW Hillen

Abstract

The thermodynamics of nucleic acids which were enclosed in reverse-phase evaporation vesicles was studied by thermal denaturation with optical recording. The denaturation curves were recorded with a dual wavelength spectrophotometer. The sum of the hypochromicity of the nucleic acid and of the change in turbidity of the vesicles was measured at 260 nm and was corrected for the change in turbidity at 320 nm. Cloned fragments of double-stranded DNA containing 180 base pairs and poly A:poly U were enclosed in REV with a yield up to every vesicle containing five nucleic acid molecules. Vesicles were prepared from egg-lecithin, and the surface charge of the vesicles was varied by addition of stearic acid, phosphatidyl-glycerol and phosphatidyl-serine. The helix-coil transition of the nucleic acid enclosed in the vesicle could be resolved from that of the free nucleic acid. Due to the enclosure into the egg-lecithin REV the transition is stabilized from 70.5 degrees to 74 degrees C, the transition is broadened from 0.7 degrees C to 2.7 degrees C. Varying the phosphatidyl-serine-lecithin-ratio from 0-100%, an optimum in the yield of enclosure at 20% was obtained, a further broadening of the transition to 5.5 degrees C and a decrease o...Continue Reading

References

Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·F Szoka, D Papahadjopoulos
Aug 9, 1977·Biochimica Et Biophysica Acta·G C Shore, J R Tata
Jul 30, 1982·Biochemical and Biophysical Research Communications·V E Imre, P L Luisi
Feb 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·C NicolauM F Juhel

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Citations

Oct 1, 1986·Journal of Biomolecular Structure & Dynamics·B Brosius, D Riesner

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