Interaction of Nup53 with Ndc1 and Nup155 is required for nuclear pore complex assembly

Journal of Cell Science
Nathalie EisenhardtWolfram Antonin

Abstract

Nuclear pore complexes (NPCs) are the gateways for nucleocytoplasmic exchange. The ordered assembly of these huge complexes from several hundred individual components into an intricate protein interaction network which deforms the two membranes of the nuclear envelope into a pore is only rudimentarily understood. Here, we show that the interaction between Nup53 and the integral pore membrane protein Ndc1 is essential for vertebrate NPC assembly. The Ndc1 binding site on Nup53 overlaps with a region that induces membrane bending and is specifically required to modulate this activity, suggesting that the membrane-deforming capability of Nup53 is adjusted during the NPC assembly process. We further demonstrate that the interaction of Nup53 and Nup155 has a crucial role in NPC formation as the main determinant of recruitment of Nup155 to the assembling pore. Overall, our results pinpoint the diversity of interaction modes accomplished by Nup53, highlighting this protein as an essential link between the pore membrane and the NPC, and as a crucial factor in the formation of the pore membrane.

References

Dec 1, 1982·The Journal of Cell Biology·L GeraceC Kondor-Koch
Apr 1, 1994·The Journal of Cell Biology·R W WozniakM P Rout
Jan 1, 1997·Annual Review of Cell and Developmental Biology·T M Gant, K L Wilson
Feb 23, 2000·The Journal of Cell Biology·M P RoutB T Chait
May 16, 2001·The Journal of Cell Biology·M MarelliR W Wozniak
Aug 28, 2002·The Journal of Cell Biology·Janet M CronshawMichael J Matunis
Feb 11, 2005·Molecular Biology of the Cell·Lisa A Hawryluk-GaraRichard W Wozniak
May 17, 2006·The Journal of Cell Biology·Fabrizia StavruDirk Görlich
Jun 17, 2006·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Corine K LauDouglass J Forbes
Sep 12, 2006·Journal of Molecular Biology·Noriko HandaShigeyuki Yokoyama
Nov 14, 2006·Proceedings of the National Academy of Sciences of the United States of America·Beth A RasalaDouglass J Forbes
Nov 30, 2007·Nature·Frank AlberMichael P Rout
Feb 8, 2008·Molecular Biology of the Cell·Lisa A Hawryluk-GaraIain W Mattaj
Jul 4, 2008·Molecular Biology of the Cell·Beth A RasalaDouglass J Forbes
Sep 10, 2008·The Journal of Cell Biology·Daniel J Anderson, Martin W Hetzer
Jan 17, 2009·Developmental Biology·Eduardo RódenasPeter Askjaer
May 6, 2009·The Journal of Cell Biology·Evgeny OnischenkoKarsten Weis
Jun 16, 2009·Molecular & Cellular Proteomics : MCP·Jeffrey A DeGrasseBrian T Chait
Aug 26, 2009·Biochemical and Biophysical Research Communications·Yusuke YamazumiTetsu Akiyama
Sep 15, 2009·Structure·Stephen G BrohawnThomas U Schwartz
Sep 29, 2009·Biochemical and Biophysical Research Communications·Barbara KindAngela Huebner
May 26, 2010·The Journal of Cell Biology·Anne ChadrinValérie Doye
Jun 23, 2010·The Journal of Cell Biology·Gandhi TheerthagiriWolfram Antonin
Jul 14, 2010·FEBS Letters·Sevil YavuzWolfram Antonin

❮ Previous
Next ❯

Citations

Jun 14, 2014·Proceedings of the National Academy of Sciences of the United States of America·Dae In KimKyle J Roux
Jan 23, 2016·Journal of Molecular Biology·Alexander von Appen, Martin Beck
Oct 17, 2015·Cellular and Molecular Life Sciences : CMLS·Daisuke Takao, Kristen J Verhey
Oct 29, 2014·BMC Biology·David A Baum, Buzz Baum
Mar 12, 2016·Cell·Kevin E Knockenhauer, Thomas U Schwartz
Sep 1, 2015·Science·Tobias StuweAndré Hoelz
Oct 2, 2015·Journal of Molecular Biology·Anna Katharina SchellhausWolfram Antonin
Sep 8, 2015·Nature Structural & Molecular Biology·Jessica FischerEd Hurt
Aug 12, 2015·Journal of Molecular Cell Biology·Liang XuYi-Han Chen
Feb 20, 2016·PLoS Biology·Samson O ObadoMichael P Rout
Jun 19, 2017·Current Opinion in Cell Biology·Ryo HayamaJavier Fernandez-Martinez
Oct 17, 2015·Cells·Justyna K LabaLiesbeth M Veenhoff
Aug 18, 2016·Journal of Cell Science·Zeinab JahedMohammad R K Mofrad
Nov 30, 2018·Protein Science : a Publication of the Protein Society·Kriti ChopraRadha Chauhan
Apr 4, 2019·Annual Review of Biophysics·Bernhard HampoelzMartin Beck
Jan 9, 2020·The FEBS Journal·Irina L RempelLiesbeth M Veenhoff
Jan 22, 2015·The Journal of Biological Chemistry·Michael LorenzWolfram Antonin
Apr 16, 2016·Science·Daniel H LinAndré Hoelz
Mar 19, 2019·Annual Review of Biochemistry·Daniel H Lin, André Hoelz
Jan 13, 2018·Nature Structural & Molecular Biology·Shotaro OtsukaJan Ellenberg
Nov 20, 2016·Journal of Cell Science·Marion Weberruss, Wolfram Antonin
Jul 19, 2016·The American Journal of Pathology·Ian A ParishJohn B Furness
Jun 6, 2015·Journal of Cell Science·Greg Kabachinski, Thomas U Schwartz
Jul 21, 2021·Biochemical Society Transactions·Alexandr A MakarovMark C Field

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.