Interaction of p53 with Mdm2 and azurin as studied by atomic force spectroscopy

Journal of Molecular Recognition : JMR
Gloria FunariSalvatore Cannistraro

Abstract

Azurin, a bacterial protein, can be internalized in cancer cells and induce apoptosis. Such anticancer effect is coupled to the formation of a complex with the tumour-suppressor p53. The mechanism by which azurin stabilizes p53 and the binding sites of their complex are still under investigation. It is also known that the predominant mechanism for p53 down-regulation implies its association to Mdm2, the main ubiquitin ligase affecting its stability. However, the p53/Mdm2 interaction, occurring at the level of both their N-terminal domains, has been characterized so far by experiments involving only partial domains of these proteins. The relevance of the p53/Mdm2 complex as a possible target of the anticancer therapies requires a deeper study of this complex as made up of the two entire proteins. Moreover, the apparent antagonist action of azurin against Mdm2, with respect of p53 regulation, might suggest the possibility that azurin binds p53 at the same site of Mdm2, preventing in such a way p53 and Mdm2 from association and thus p53 from degradation. By following the interaction of the two entire proteins by atomic force spectroscopy, we have assessed the formation of a specific complex between p53 and Mdm2. We found for it a ...Continue Reading

References

Jul 1, 1993·Molecular and Cellular Biology·J ChenA J Levine
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·P HinterdorferH Schindler
May 1, 1996·Genes & Development·L J Ko, C Prives
Apr 1, 1997·Biophysical Journal·E Evans, K Ritchie
May 15, 1997·Nature·Y HauptM Oren
May 15, 1997·Nature·M H KubbutatK H Vousden
Mar 5, 1999·Cellular and Molecular Life Sciences : CMLS·D A FreedmanA J Levine
Apr 12, 2000·Proceedings of the National Academy of Sciences of the United States of America·W BaumgartnerD Drenckhahn
Oct 14, 2000·Archives of Biochemistry and Biophysics·Z LaiR A Copeland
Dec 1, 2000·Nature·B VogelsteinA J Levine
Dec 7, 2000·Progress in Biophysics and Molecular Biology·J ZlatanovaS H Leuba
Apr 20, 2002·Biophysical Journal·Susanne Wielert-BadtRolf K-H Kinne
Oct 9, 2002·Journal of Molecular Biology·Stefan BellJohannes Buchner
Oct 17, 2002·Journal of Molecular Biology·Oliver SchonAlan R Fersht
Oct 24, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tohru YamadaAnanda M Chakrabarty
Dec 31, 2002·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Matthias Rief, Helmut Grubmüller
Jan 11, 2003·The Journal of Biological Chemistry·William HanleyKonstantinos Konstantopoulos
Apr 30, 2003·Cell Death and Differentiation·M Oren
Sep 23, 2003·Journal of Molecular Biology·Roger DawsonJohannes Buchner
Jan 6, 2004·Science·Lyubomir T VassilevEmily A Liu
Mar 26, 2004·Biophysical Journal·Timothy V RattoMichael W McElfresh
Nov 24, 2004·Biophysical Journal·Bryan T MarshallCheng Zhu
Apr 20, 2005·Oncogene·Sandra L Harris, Arnold J Levine
May 26, 2005·Biochemical and Biophysical Research Communications·David Apiyo, Pernilla Wittung-Stafshede
Sep 15, 2005·The Journal of Biological Chemistry·Seung-Wook ChiKyou-Hoon Han
Nov 5, 2005·Proceedings of the National Academy of Sciences of the United States of America·Todd A SulchekAleksandr Noy
Dec 24, 2005·Journal of Cell Science·Porntula PanorchanDenis Wirtz
Feb 1, 2006·Molecular Cancer Research : MCR·Wan Mui ChanRandy Y C Poon
Mar 25, 2006·Cell Death and Differentiation·K G Wiman
Apr 4, 2006·Biophysical Journal·Todd SulchekAleksandr Noy
Apr 8, 2006·Cell Death and Differentiation·M F Lavin, N Gueven
Apr 22, 2006·Nature Methods·Peter Hinterdorfer, Yves F Dufrêne
Oct 7, 2006·Colloids and Surfaces. B, Biointerfaces·Esben ThormannOle G Mouritsen
Nov 28, 2006·Trends in Molecular Medicine·Lyubomir T Vassilev
Aug 19, 2007·Journal of Molecular Recognition : JMR·Valentina De GrandisSalvatore Cannistraro
Dec 22, 2007·Langmuir : the ACS Journal of Surfaces and Colloids·Tong Seng LimChwee Teck Lim
Feb 1, 2008·Cell Cycle·Hilary V CleggYanping Zhang
Feb 6, 2008·Journal of Molecular Recognition : JMR·Monia TarantaSalvatore Cannistraro

❮ Previous
Next ❯

Citations

Oct 7, 2011·International Journal of Nanomedicine·Fabio DomeniciSalvatore Cannistraro
Dec 14, 2011·International Journal of Nanomedicine·Anna Rita BizzarriSalvatore Cannistraro
May 18, 2011·Journal of Molecular Recognition : JMR·Fabio DomeniciSalvatore Cannistraro
Feb 6, 2013·Biochemistry. Biokhimii︠a︡·I V SafenkovaB B Dzantievf
Jul 17, 2010·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xavier SisquellaXavier Fernàndez-Busquets
Jun 27, 2019·International Journal of Molecular Sciences·Sara SignorelliAnna Rita Bizzarri
Sep 18, 2014·Chemical Biology & Drug Design·Zhenzhen LiuMinyong Li
Dec 18, 2020·Sensors·Anna Rita Bizzarri, Salvatore Cannistraro
Oct 27, 2020·The Journal of Physical Chemistry. B·Anna Rita Bizzarri, Salvatore Cannistraro

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Apoptosis in Cancer

Apoptosis is an important mechanism in cancer. By evading apoptosis, tumors can continue to grow without regulation and metastasize systemically. Many therapies are evaluating the use of pro-apoptotic activation to eliminate cancer growth. Here is the latest research on apoptosis in cancer.