Interaction of packaging motor with the polymerase complex of dsRNA bacteriophage

Virology
Jirí LísalRoman Tuma

Abstract

Many viruses employ molecular motors to package their genomes into preformed empty capsids (procapsids). In dsRNA bacteriophages the packaging motor is a hexameric ATPase P4, which is an integral part of the multisubunit procapsid. Structural and biochemical studies revealed a plausible RNA-translocation mechanism for the isolated hexamer. However, little is known about the structure and regulation of the hexamer within the procapsid. Here we use hydrogen-deuterium exchange and mass spectrometry to delineate the interactions of the P4 hexamer with the bacteriophage phi12 procapsid. P4 associates with the procapsid via its C-terminal face. The interactions also stabilize subunit interfaces within the hexamer. The conformation of the virus-bound hexamer is more stable than the hexamer in solution, which is prone to spontaneous ring openings. We propose that the stabilization within the viral capsid increases the packaging processivity and confers selectivity during RNA loading.

References

Feb 1, 1996·Current Opinion in Structural Biology·S W EnglanderL Mayne
Oct 13, 1998·Mass Spectrometry Reviews·A G MarshallG S Jackson
Sep 11, 2003·The Journal of Biological Chemistry·Denis E KainovEugene V Makeyev
Mar 3, 2004·Acta Crystallographica. Section D, Biological Crystallography·Erika J ManciniJonathan M Grimes
Mar 11, 2004·Virus Research·Minna M Poranen, Roman Tuma
Apr 20, 2004·Current Opinion in Structural Biology·Jason Lanman, Peter E Prevelige
Jun 23, 2004·Nature Structural & Molecular Biology·Jason LanmanPeter E Prevelige
Apr 19, 2005·Nature Structural & Molecular Biology·Jirí LísalRoman Tuma
Apr 21, 2005·The Journal of Biological Chemistry·Jirí Lísal, Roman Tuma

❮ Previous
Next ❯

Citations

Nov 2, 2012·Journal of Virology·Eiko Matsuo, Polly Roy
May 5, 2012·Protein Expression and Purification·Ansgar Brock
Feb 2, 2011·Current Opinion in Structural Biology·Artem Y LyubimovJames M Berger
Mar 28, 2007·Current Opinion in Structural Biology·Juha T Huiskonen, Sarah J Butcher
May 24, 2008·Mass Spectrometry Reviews·Victoria L MortonAlison E Ashcroft
Nov 11, 2006·Journal of Structural Biology·Juha T HuiskonenSarah J Butcher
Apr 14, 2016·Methods : a Companion to Methods in Enzymology·Sean M Carney, Michael A Trakselis
Aug 21, 2014·Biophysical Journal·Gaël RadouEmanuele Paci
May 10, 2008·Analytical Chemistry·Tanner M SchaubAlan G Marshall

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.