Oct 1, 1989

Interaction of paramyxoviruses with human basophils and their effect on histamine release

The Journal of Allergy and Clinical Immunology
F Sanchez-Legrand, T F Smith

Abstract

We have demonstrated that human peripheral blood basophils released histamine on direct incubation with paramyxoviruses in vitro. Most histamine release occurred during the first 15 to 30 minutes after challenge, depending on the dose of virus used; release initiated by virus was complete by 1 hour. At a virus/cell ratio of 1:1, Sendai virus caused 41 +/- 9% histamine release, whereas parainfluenza type 3 (PI-3) virus caused 25 +/- 5% release and respiratory syncytial (RS) virus caused 19 +/- 5% release. Sendai, but not PI-3 or RS, also caused a decrease in cell number and release of lactic dehydrogenase; however, this apparent cell lysis did not account for all the histamine released. Incubation of cells with virus desensitized them to subsequent triggering by viruses but did not affect response of cells to other stimuli. Histamine release was dependent on the virus/cell ratio, temperature, and metabolic energy, but it was not strictly dependent on the presence of calcium in the extracellular medium. Histamine release was not affected by preincubation of cells with colchicine, suggesting that microtubules were not involved in the release process. Basophils desensitized by anti-IgE in the absence of calcium or treated with lact...Continue Reading

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Mentioned in this Paper

Pathologic Cytolysis
Histamine Measurement
Metabolic Process, Cellular
Respiratory Syncytial Virus Infections
Anti-Immunoglobulin E antibody
Calcium [EPC]
Basophils
Extracellular
Calcium
SpecimenType - Basophils

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