Interaction of parathyroid hormone with membranes of kidney cortex: degradation of the hormone and activation of adenylate cyclase.

Endocrinology
L L ChuD V Cohn

Abstract

Plasma membranes prepared from rat renal cortex contain both a parathyroid hormone-sensitive adenylate cyclase and a potent proteolytic activity which degrades the hormone into peptide fragments. The degree and pattern of degradation was determined by subjecting incubation mixtures to gel filtration and ion exchange chromatography. Estimation of the degree of degradation by acid precipitation of the intact hormone was inadequate since metabolism of the hormone apparently generated acid-insoluble fragments. When parathyroid hormone was incubated with membrane fraction, the capacity of its stimulatory effect on adenylate cyclase decreased steadily. This decrease of PTH activitiy could be closely related to the degradation of intact hormone by the same membrane preparation. The adenylate cyclase and degradative activity appeared to exist in similar membrane structures since they could not be separated by centrifugation through sucrose density gradients. The degradation of the hormone could not be inhibited by Trasylol and pancreatic or soybean trypsin inhibitors and was only slightly inhibited by ribonuclease and benzamidine. Histone (1 mg per ml), on the other hand, was able to decrease the degradation of the hormone and prevent ...Continue Reading

Citations

Dec 1, 1977·The American Journal of Anatomy·D M Biddulph, R W Wrenn
Mar 1, 1991·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J J OrloffA F Stewart
May 16, 1979·Biochimica Et Biophysica Acta·A A BialasiewiczR D Hesch
Nov 15, 1979·The New England Journal of Medicine·K J MartinE Slatopolsky
Oct 1, 1977·The Journal of Clinical Investigation·K J MartinE Slatopolsky
Sep 1, 1976·FEBS Letters·H Jüppner, R D Hesch
Aug 1, 1989·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·G A NickolsC W Cooper
Aug 1, 1978·Metabolism: Clinical and Experimental·S B OldhamF R Singer
Mar 18, 2020·Clinica Chimica Acta; International Journal of Clinical Chemistry·Stan R UrsemAnnemieke C Heijboer
Apr 1, 1979·The American Journal of Physiology·G A NickolsL R Forte
Jun 6, 1977·Biochemical and Biophysical Research Communications·C MinkinM Walling

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