Interaction of PARP-2 with AP site containing DNA

Biochimie
Mikhail M KutuzovOlga Lavrik

Abstract

In eukaryotes the stability of genome is provided by functioning of DNA repair systems. One of the main DNA repair pathways in eukaryotes is the base excision repair (BER). This system requires precise regulation for correct functioning. Two members of the PARP family - PARP-1 and PARP-2, which can be activated by DNA damage - are widely considered as regulators of DNA repair processes, including BER. In contrast to PARP-1, the role of PARP-2 in BER has not been extensively studied yet. Since AP site is one of the most frequent type of DNA damage and a key intermediate of BER at the stage preceding formation of DNA breaks, in this paper we focused on the characterization of PARP-2 interaction with AP site-containing DNAs. We demonstrated that PARP-2, like PARP-1, can interact with the intact AP site via Schiff base formation, in spite of crucial difference in the structure of the DNA binding domains of these PARPs. By cross-linking of PARPs to AP DNA, we determined that the N-terminal domains of both PARPs are involved in formation of cross-links with AP DNA. We have also confirmed that DNA binding by PARP-2, in contrast to PARP-1, is not modulated by autoPARylation. PARP-2, like PARP-1, can inhibit the activity of APE1 by bind...Continue Reading

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Citations

May 1, 2016·Biochimica Et Biophysica Acta·Anastasiya A KosovaOlga I Lavrik
Nov 2, 2016·Nucleic Acids Research·Ibtissam TalhaouiAlexander A Ishchenko
Oct 7, 2016·Scientific Reports·Ezeogo ObajiLari Lehtiö
May 10, 2017·Chemical Research in Toxicology·Suzanne J Admiraal, Patrick J O'Brien
Oct 16, 2018·Nucleic Acids Research·Ezeogo ObajiLari Lehtiö
Sep 30, 2016·Biochemistry. Biokhimii︠a︡·N S DyrkheevaO I Lavrik
Feb 13, 2020·Cells·Laura JankóPéter Bai
May 18, 2020·DNA Repair·Petria S Thompson, David Cortez
Jun 4, 2020·DNA Repair·Svetlana Khodyreva, Olga Lavrik
Jul 15, 2020·DNA Repair·Katherine M Amidon, Brandt F Eichman
May 1, 2021·International Journal of Molecular Sciences·Inna Vasil'evaOlga Lavrik
Aug 28, 2021·Progress in Lipid Research·Magdolna SzántóPeter Bai

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