Interaction of peptides with biomembranes assessed by potential-sensitive fluorescent probes

Journal of Peptide Science : an Official Publication of the European Peptide Society
Pedro M MatosNuno C Santos

Abstract

Peptide-membrane interaction is an important step to be evaluated in a study of the activity and mode of action of several bioactive peptides. A variety of methods are available; however, few of them satisfy the criteria of being sensitive, biocompatible, versatile, easy to perform, and allowing real-time monitoring as the use of potential-sensitive fluorescent probes. Here we review methods for detecting the effects of membrane-active peptides, even those that are not intrinsically fluorescent, on the different types of membrane potentials, with a special emphasis on studies conducted with living cells. FPE is a probe sensitive to surface potential and detects electrostatic interactions at the water-lipid interface. Di-8-ANEPPS is sensitive to dipole potential and detects membrane incorporations. Transmembrane potential changes reveal major membrane destabilizations, such as in pore formation. The combination of the information obtained from the three potential variations can lead to a more elucidative picture of the mechanisms of the interaction of relevant peptides with biomembranes.

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Citations

Apr 15, 2010·Journal of Fluorescence·Magda PrzybyloMarek Langner
Apr 6, 2011·PloS One·Filomena A CarvalhoNuno C Santos
Aug 2, 2015·Chemistry and Physics of Lipids·Manuela DenzPeter Müller
Nov 16, 2010·Biochemical and Biophysical Research Communications·Pedro M MatosNuno C Santos
May 8, 2014·Biochimica Et Biophysica Acta·Arunima Chaudhuri, Amitabha Chattopadhyay
Jul 22, 2010·Biochimica Et Biophysica Acta·Pedro M MatosNuno C Santos
Aug 24, 2018·Biomolecules·Fatma Gizem AvciElif Ozkirimli
Mar 20, 2008·Journal of Peptide Science : an Official Publication of the European Peptide Society·Miguel A R B Castanho, Margitta Dathe
Jan 2, 2014·American Journal of Physiology. Renal Physiology·Zhong ZhangThomas L Pallone
Jul 16, 2009·Molecular BioSystems·Raz Jelinek, Liron Silbert
Feb 8, 2013·Organic & Biomolecular Chemistry·Marta Dal Molin, Stefan Matile
Nov 12, 2020·International Journal of Molecular Sciences·Francisca Lidiane Linhares de AguiarSónia Gonçalves
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Aug 13, 2011·Molecular Pharmaceutics·Marta M B RibeiroMiguel A Castanho
Sep 4, 2021·Biochimica Et Biophysica Acta. Biomembranes·Patrícia M SilvaNuno C Santos

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