PMID: 9445393Mar 7, 1998Paper

Interaction of phosphatidic acid and phosphatidylserine with the Ca2+-ATPase of sarcoplasmic reticulum and the mechanism of inhibition

The Biochemical Journal
K A DaltonA G Lee

Abstract

The sarcoplasmic reticulum of skeletal muscle contains anionic phospholipids as well as the zwitterionic phosphatidylcholine and phosphatidylethanolamine. Here we study the effects of anionic phospholipids on the activity of the Ca2+-ATPase purified from the membrane. Reconstitution of the Ca2+-ATPase into dioleoylphosphatidylserine [di(C18:1)PS] or dioleoylphosphatidic acid [di(C18:1)PA] leads to a decrease in ATPase activity. Measurements of the quenching of the tryptophan fluorescence of the ATPase by brominated phospholipids give a relative binding constant for the anionic lipids compared with dioleoylphosphatidylcholine close to 1 and suggest that phosphatidic acid only binds to the ATPase at the bulk lipid sites around the ATPase. Addition of di(C18:1)PS or di(C18:1)PA to the ATPase in the short-chain dimyristoleoylphosphatidylcholine [di(C14:1)PC] reverse the effects of the short-chain lipid on ATPase activity and on Ca2+ binding, as revealed by the response of tryptophan fluorescence intensity to Ca2+ binding. It is concluded that the lipid headgroup and lipid fatty acyl chains have separate effects on the function of the ATPase. The anionic phospholipids have no significant effect on Ca2+ binding to the ATPase; the lev...Continue Reading

Citations

Jul 4, 2012·Current Opinion in Structural Biology·Lea Thøgersen, Poul Nissen
Aug 12, 2008·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Peter VossLubica Horakova
Jul 22, 2015·The Journal of Membrane Biology·Val Andrew FajardoA Russell Tupling
May 30, 2006·Biochimica Et Biophysica Acta·Jochen Zimmer, Declan A Doyle
Feb 26, 2008·Biochimica Et Biophysica Acta·Derek Marsh
Mar 12, 2013·Chemistry and Physics of Lipids·Elizabeth J Denning, Oliver Beckstein
Aug 2, 2006·Molecular BioSystems·Anthony G Lee
Oct 5, 2013·Journal of Enzyme Inhibition and Medicinal Chemistry·Petronela ŽižkováLubica Horáková
Sep 25, 2019·Biomolecules·Louise Conrard, Donatienne Tyteca
Mar 28, 2003·The Journal of Biological Chemistry·Claudia V Filomatori, Alcides F Rega
Sep 24, 2002·The Journal of Biological Chemistry·Yasuo OkamotoYusuf A Hannun
Jan 25, 2017·The Journal of Biological Chemistry·Nawal KassasNicolas Vitale
Feb 4, 1999·Biochemistry·G W HunterT C Squier

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