PMID: 9556348Apr 7, 1998Paper

Interaction of phospholipid vesicles with smooth metal-oxide surfaces

Biochimica Et Biophysica Acta
G Csúcs, J J Ramsden

Abstract

The interaction of phospholipid vesicles with planar metal oxide supports has been previously reported as a means of preparing supported lipid bilayers, which are useful models of biological membranes. Nevertheless, extant evidence that bilayers are actually formed is rather circumstantial, and the necessary and sufficient conditions for their formation have never been delineated. Here, we tackle this problem by using smooth planar optical waveguides as the support. Analysis of the lightmode spectra of the waveguides, measured in situ during the deposition process, yields the mass of lipid deposited at the solid/liquid interface. By comparing the optogeometric parameters of the structures assembled from the vesicles with those of a lipid bilayer of known structure assembled using the Langmuir-Blodgett technique, we show that in many cases the vesicles remain intact and form a supported layer of vesicles rather than a bilayer, and often mixed structures (intact vesicles embedded in a bilayer partially covering the surface) occur. Careful analysis of the lipid deposition kinetics corroborates this result. We have also found that divalent cations dramatically promote attachment of mixed phosphatidylcholine/phosphatidylglycerol ves...Continue Reading

References

Jul 14, 1982·Biochimica Et Biophysica Acta·S Ohki
Feb 15, 1995·Biochimica Et Biophysica Acta·I VikholmO Teleman

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Citations

Nov 15, 2011·Analytical and Bioanalytical Chemistry·L StellerR Salzer
Apr 14, 2011·Biomedical Microdevices·Bala Murali VenkatesanRashid Bashir
Jun 29, 2004·Micron : the International Research and Review Journal for Microscopy·Mohammad A Hussain, Christopher A Siedlecki
Jul 12, 2002·Biomaterials·J VörösN D Spencer
Jan 18, 2006·Journal of Colloid and Interface Science·Seiichi MoritaRyoichi Kuboi
Jan 27, 2005·Biochimica Et Biophysica Acta·Hanna P VacklinR K Thomas
Feb 11, 2015·Biomacromolecules·Qasim SaleemPeter M Macdonald
Jan 31, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Michael S KesslerSusan D Gillmor
Nov 10, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Julia A BraungerClaudia Steinem
Jun 1, 2008·Biointerphases·Christoph MerzErik Reimhult
Sep 29, 2004·Biomaterials·M P HeubergerN D Spencer
Sep 23, 2014·Colloids and Surfaces. B, Biointerfaces·Quoc-Chon LeBernard Humbert
Jun 7, 2014·Applied Biochemistry and Biotechnology·Mohanlal Bhuvana, Venkataraman Dharuman
Jul 20, 2001·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·T R Weikl, R Lipowsky
Nov 5, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Srinivas ParimiClive A Prestidge
Sep 13, 2008·Langmuir : the ACS Journal of Surfaces and Colloids·Ryugo TeroTsuneo Urisu
Nov 15, 2006·Surface Science Reports·Edward T Castellana, Paul S Cremer
Sep 23, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Nam-Joon Cho, Curtis W Frank
Jan 18, 2020·Langmuir : the ACS Journal of Surfaces and Colloids·Jonas SchäferClaudia Steinem

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