Interaction of pituitary hormones and expression of clock genes modulated by bone morphogenetic protein-4 and melatonin

Biochemical and Biophysical Research Communications
Naoko Tsukamoto-YamauchiFumio Otsuka

Abstract

Functional interaction of clock genes and pituitary hormones was investigated by focusing on bone morphogenetic protein (BMP)-4 and melatonin actions in anterior pituitary cells. A significant correlation between the mRNA expression of proopiomelanocortin (POMC) and Per2 was revealed in serial cultures of corticotrope AtT20 cells. Knockdown of Per2 expression by siRNA in AtT20 cells resulted in a significant reduction of POMC mRNA level with or without corticotropin-releasing hormone (CRH) stimulation. Treatments with BMP-4 and melatonin, both of which suppress POMC expression, reduced Per2 mRNA as well as protein levels in AtT20 cells. On the other hand, in lactosomatotrope GH3 cells, an expressional correlation was found between prolactin (PRL) and Clock mRNA levels, which was attenuated in the presence of forskolin treatment. The siRNA-mediated knockdown of Clock expression, but not that of Bmal1, significantly reduced PRL mRNA levels in GH3 cells. Interestingly, Clock mRNA and protein levels did not fluctuate with melatonin, BMP-4 or forskolin treatment, although Bmal1 expression was significantly increased by forskolin treatment. Collectively, a significant correlation between the expression of POMC and Per2 and that betwe...Continue Reading

References

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Citations

May 1, 2016·Biochemical and Biophysical Research Communications·Tao ShuLimin Rong
Mar 27, 2018·European Journal of Clinical Investigation·Anna AngelousiGregory Kaltsas
Apr 9, 2019·Endocrine-related Cancer·Anna AngelousiGeorge P Chrousos
Apr 2, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Jaqueline Moraes BazioliJoão Ernesto de Carvalho
Sep 19, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiaopeng SongLi Gao
May 1, 2021·International Journal of Molecular Sciences·Satoshi FujisawaFumio Otsuka

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