PMID: 9557638Apr 29, 1998Paper

Interaction of poliovirus with its purified receptor and conformational alteration in the virion

Journal of Virology
M AritaA Nomoto

Abstract

Polypeptides of amino acids 1 to 241 (PVR241) and 1 to 330 (PVR330) of the human poliovirus receptor (hPVR) were produced in a baculovirus expression system. PVR241 contained extracellular domains 1 and 2 of hPVR, and PVR330 contained extracellular domains 1, 2, and 3. These peptides were purified by immunoaffinity column chromatography with an anti-hPVR monoclonal antibody (MAb). After the purification, PVR241 and PVR330 appeared to retain their native conformation as judged by reactivity with an anti-PVR MAb that recognized domain 1 of hPVR in a conformation-dependent manner. The virulent Mahoney strain of poliovirus type 1 was mixed with the purified PVRs in various concentrations. An average of at least 43 PVR330 molecules were able to bind to one virion particle under the conditions used. The equilibrium dissociation constant between the PVR330 molecule and the PVR binding site (canyon) on the virion was determined to be 4.50 +/- (0.86) x 10(-8) M at 4 degrees C. Higher rates of conformational change of the virus (160S) to 135S and 80S particles were observed as the concentration of PVR330 was increased. In this in vitro system, the ratio of the amount of the 135S particle to that of the 80S particle seemed to be always co...Continue Reading

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Citations

May 29, 2004·The Journal of General Virology·Magdalena T TostesonMarie Chow
Feb 7, 2007·Intervirology·Elizabeth González DuránJuan Santiago Salas Benito
Oct 15, 2010·Journal of Virology·Payel Sarkar, Pranav Danthi
Jun 5, 2010·Future Microbiology·Craig E CameronIbrahim M Moustafa
Apr 20, 2012·Journal of Virology·Jani J T SeitsonenSarah J Butcher
Dec 20, 2013·Journal of Virology·Ke LyuRong Chen
Jun 16, 2005·Journal of Virology·Stephen BerrymanTerry Jackson
Oct 28, 2004·Journal of Virology·E Susanne JohanssonDarren R Shafren
Mar 12, 2010·Uirusu·Satoshi Koike
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Mar 29, 2003·Journal of Virology·Yongning HeMichael G Rossmann
May 17, 2000·The Journal of General Virology·J Schneider-Schaulies
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Jul 12, 2005·Structure·Chuan XiaoMichael G Rossmann

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