Interaction of polyethylene glycol with cytochrome c investigated via in vitro and in silico approaches.

Scientific Reports
Zahoor Ahmad ParrayAsimul Islam


One of the significant proteins that have attracted research groups due to virtue of being a potent selective anticancer drug target and property of triggering apoptosis upon release in cytoplasm is cytochrome c (cyt c). The mechanical transformations due to the macromolecular crowding in membrane in the mammalian cell are proposed to be useful inductors of changes in volume. It is very interesting to know that mitochondrial function were observed to be improved by polyethylene glycol (PEG) interaction, which in turn inhibits the cyt c (a pro-apoptotic cell death factor). In this work, the effect of polyethylene glycol of molecular weight 4 kilo Dalton (PEG 4 kDa) was investigated to highlight the structural transformations (tertiary and secondary structure) in cyt c using a choice of spectroscopic techniques (including UV-Vis absorption, near-UV, far-UV and Soret circular dichroism and fluorescence spectroscopy), which shows noteworthy shifts in the secondary and tertiary structures at higher concentrations of PEG 4 kDa with small changes in the heme-globular interactions. The size distribution changes of native protein treated with various concentrations of the crowder were observed and analyzed by dynamic light scattering (D...Continue Reading


Apr 10, 1990·Biochemistry·Y GotoA L Fink
Jul 20, 1990·Journal of Molecular Biology·G W BushnellG D Brayer
Jan 1, 1986·Advances in Protein Chemistry·S Krimm, J Bandekar
Jan 1, 1985·Annual Review of Biochemistry·J M Beechem, L Brand
Nov 19, 1985·Biochemistry·T Arakawa, S N Timasheff
Mar 27, 1973·Biochemistry·J D MorrisettA M Gotto
Jan 1, 1972·Methods in Enzymology·Y Nozaki
Dec 1, 1965·Proceedings of the National Academy of Sciences of the United States of America·H A HarburyS N Vinogradov
Apr 15, 1982·Archives of Biochemistry and Biophysics·P M Mulqueen, M J Kronman
Jan 1, 1995·Bioconjugate Chemistry·C MonfardiniF M Veronese
May 1, 1996·Proteins·V SobolevM Edelman
Nov 14, 1997·Journal of Inorganic Biochemistry·Roberto Santucci, F Ascoli
May 20, 1999·Journal of Protein Chemistry·L Gebicka, J L Gebicki
Dec 23, 2000·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·M B Burg
Oct 9, 2002·Current Protein & Peptide Science·S M Kelly, N C Price
May 13, 2003·Journal of Protein Chemistry·Ali Akbar Moosavi-MovahediN Sheibani
May 16, 2003·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Federica SinibaldiRoberto Santucci
Mar 1, 1959·The Biochemical Journal·E MARGOLIASH, N FROHWIRT
Nov 25, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Beatriz FarruggiaGuillermo Picó
Jun 9, 2005·Methods in Molecular Biology·Karin Nienhaus, G Ulrich Nienhaus
Oct 8, 2005·Science·Diana SpieringsDouglas R Green
Jan 13, 2006·Nature Chemical Biology·Valerian E KaganGregory G Borisenko
Aug 15, 2006·Nature Structural & Molecular Biology·Nataliya PopovychCharalampos G Kalodimos
May 18, 2007·Journal of Biochemistry·Sadaf Fatima, Rizwan Hasan Khan
Aug 10, 2007·Acta Biochimica Et Biophysica Sinica·Jilie Kong, Shaoning Yu
Aug 12, 2008·Biomedical Materials·M-E Aubin-Tam, K Hamad-Schifferli
Mar 12, 2009·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Md Khurshid Alam KhanFaizan Ahmad
May 30, 2009·Luminescence : the Journal of Biological and Chemical Luminescence·Jianniao TianShulin Zhao
May 4, 2010·The Journal of Physical Chemistry. B·Mrityunjoy MahatoG B Talapatra
Jul 10, 2010·Proceedings of the National Academy of Sciences of the United States of America·Shun HirotaYoshiki Higuchi
Mar 25, 2011·Metallomics : Integrated Biometal Science·Md Khurshid Alam KhanFaizan Ahmad
Mar 21, 2013·Journal of Controlled Release : Official Journal of the Controlled Release Society·Qingrui HuangTao Hu
Apr 26, 2013·Cellular and Molecular Life Sciences : CMLS·Sobia ZaidiFaizan Ahmad
Jun 12, 2013·Biochimica Et Biophysica Acta·Krishna Rajarathnam, Jörg Rösgen
Dec 3, 2014·Methods : a Companion to Methods in Enzymology·Georg Krainer, Sandro Keller
Jan 15, 2015·Journal of Biomolecular Structure & Dynamics·Abdullah NaiyerFaizan Ahmad
Jan 21, 2015·Cell·Marisa M MerinoEduardo Moreno
Jan 27, 2015·Methods in Molecular Biology·Sargis Dallakyan, Arthur J Olson
May 3, 2015·Carbohydrate Polymers·Settimio PacelliMaria Antonietta Casadei
Jul 6, 2016·International Journal of Biological Macromolecules·Sabab Hasan KhanFaizan Ahmad
Mar 1, 2017·International Journal of Biological Macromolecules·Zahoor Ahmad ParrayAsimul Islam
Aug 16, 2017·International Journal of Biological Macromolecules·Khalida NasreenAsimul Islam
Mar 16, 2018·Biochimica Et Biophysica Acta. General Subjects·Wataru SatoKoichiro Ishimori
Dec 27, 2018·International Journal of Biological Macromolecules·Zahoor Ahmad ParrayAsimul Islam
May 28, 2014·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Jiang WuJie Zheng
Aug 19, 2020·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Zahoor Ahmad ParrayAsimul Islam

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