Interaction of Positively Charged Gold Nanoparticles with Cancer Cells Monitored by an in Situ Label-Free Optical Biosensor and Transmission Electron Microscopy

ACS Applied Materials & Interfaces
Beatrix PeterRobert Horvath

Abstract

Functionalized nanoparticles (NPs) can penetrate into living cells and vesicles, opening up an extensive range of novel directions. For example, NPs are intensively employed in targeted drug delivery and biomedical imaging. However, the real-time kinetics and dynamics of NP-living cell interactions remained uncovered. In this study, we in situ monitored the cellular uptake of gold NPs-functionalized with positively charged alkaline thiol-into surface-adhered cancer cells, by using a high-throughput label-free optical biosensor employing resonant waveguide gratings. The characteristic kinetic curves upon NP exposure of cell-coated biosensor surfaces were recorded and compared to the kinetics of NP adsorption onto bare sensor surfaces. We demonstrated that from the above kinetic information, one can conclude about the interactions between the living cells and the NPs. Real-time biosensor data suggested the cellular uptake of the functionalized NPs by an active process. It was found that positively charged particles penetrate into the cells more effectively than negatively charged control particles, and the optimal size for the cellular uptake of the positively charged particles is around 5 nm. These conclusions were obtained in a...Continue Reading

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Citations

Oct 22, 2019·Chembiochem : a European Journal of Chemical Biology·Sandeep KaushalDong Kee Yi
Feb 26, 2020·Scientific Reports·Márta Lídia DebreczeniRobert Horvath
May 14, 2021·Journal of Colloid and Interface Science·Kinga Dóra KovácsRobert Horvath
Jan 18, 2019·Langmuir : the ACS Journal of Surfaces and Colloids·Andras SafticsRobert Horvath
Sep 22, 2018·Langmuir : the ACS Journal of Surfaces and Colloids·Xueqing XingZhonghua Wu
Nov 28, 2021·Biologia Futura·Kinga MolnárAndrás Dinnyés

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