Interaction of processes on different length scales in a bioelastomer capable of performing energy conversion

Biopolymers
M MannoD W Urry

Abstract

This work concerns the aggregation properties of (Gly-Val-Gly-Val-Pro)(251) rec, a polypentapeptide reflecting a highly conserved repetitive unit of the bioelastomer, elastin. On raising the temperature of aqueous solutions above 25 degrees C, this polypeptide was already known to undergo concurrent conformational changes (hydrophobic folding), phase separation, and self-assembly with formation of aggregated three-stranded filaments composed of dynamic polypeptide helices, called beta-spirals. Aggregates obtained from the solution can be shaped into bands that acquire entropic elastic properties upon gamma-irradiation and can perform a variety of energy conversions. Previous studies have shown that aggregation is prompted by the (diverging) critical fluctuations of concentration occurring in the solution, in vicinity of its spinodal line. Here, we present combined circular dicroism (CD) and light scattering experiments, and independent fittings of experimental data to the theoretical spinodal and binodal (coexistence) lines. Results show the following logical and causal sequence of processes: (a) Smooth and progressive conformational changes promoted by concentration fluctuations occurring as temperature is raised "pull down" (...Continue Reading

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Citations

Apr 4, 2007·Pharmaceutical Research·Mohamed HaiderKam W Leong
May 15, 2009·Physical Chemistry Chemical Physics : PCCP·Rita CarrottaPier Luigi San Biagio
Mar 26, 2002·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·D W UrryT Parker
May 3, 2007·Journal of Biomaterials Science. Polymer Edition·J Carlos Rodríguez-CabelloArtur Ribeiro
Mar 13, 2014·Langmuir : the ACS Journal of Surfaces and Colloids·Guillermo Pinedo-MartínJ Carlos Rodríguez-Cabello
Jun 13, 2006·Drug Delivery·T Cooper Woods, Dan W Urry
Dec 26, 2015·Advanced Drug Delivery Reviews·José Carlos Rodriguez-CabelloAlessandra Girotti
Jul 27, 2010·Advanced Drug Delivery Reviews·Dan W UrryMichal Nowicki
Jan 18, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·R Herrero-VanrellJ C Rodríguez-Cabello
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Jul 8, 2009·Biophysical Journal·Artur RibeiroJ Carlos Rodríguez-Cabello
Jul 15, 2016·The Journal of Physical Chemistry. B·Olga MatsarskaiaFrank Schreiber
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Nov 23, 2006·Journal of the American Chemical Society·Yuni JungLiviu Movileanu
Oct 14, 2004·Journal of the American Chemical Society·Javier RegueraJosé Carlos Rodríguez-Cabello
May 14, 2003·Biomacromolecules·Dana KurkováMatilde Alonso

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