Interaction of protein inhibitor of activated STAT (PIAS) proteins with the TATA-binding protein, TBP

The Journal of Biological Chemistry
Justin R Prigge, Edward E Schmidt

Abstract

Transcription activators often recruit promoter-targeted assembly of a pre-initiation complex; many repressors antagonize recruitment. These activities can involve direct interactions with proteins in the pre-initiation complex. We used an optimized yeast two-hybrid system to screen mouse pregnancy-associated libraries for proteins that interact with TATA-binding protein (TBP). Screens revealed an interaction between TBP and a single member of the zinc finger family of transcription factors, ZFP523. Two members of the protein inhibitor of activated STAT (PIAS) family, PIAS1 and PIAS3, also interacted with TBP in screens. Endogenous PIAS1 and TBP co-immunoprecipitated from nuclear extracts, suggesting the interaction occurred in vivo. In vitro-translated PIAS1 and TBP co-immunoprecipitated, which indicated that other nuclear proteins were not required for the interaction. Deletion analysis mapped the PIAS-interacting domain of TBP to the conserved TBP(CORE) and the TBP-interacting domain on PIAS1 to a 39-amino acid C-terminal region. Mammals issue seven known PIAS proteins from four pias genes, pias1, pias3, piasx, and piasy, each with different cell type-specific expression patterns; the TBP-interacting domain reported here is ...Continue Reading

References

Apr 11, 1995·Proceedings of the National Academy of Sciences of the United States of America·J DeJongR G Roeder
Jul 1, 1993·Genes & Development·N Hernandez
Feb 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·H TangR H Ebright
May 10, 1996·Science·J H GeigerP B Sigler
Aug 5, 1996·Biochemical and Biophysical Research Communications·T OhbayashiT Tamura
Nov 8, 1996·The Journal of Biological Chemistry·D L SwopeJ C Chrivia
Aug 15, 1998·The Journal of Biological Chemistry·E MyslinskiP Carbon
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·B LiuK Shuai
Jan 28, 1999·The Journal of Biological Chemistry·A M MoilanenJ J Palvimo
Jun 15, 2000·Journal of Molecular Neuroscience : MN·S SturmF A White
Sep 12, 2000·Nature Cell Biology·F Melchior, L Hengst
Mar 15, 2001·Proceedings of the National Academy of Sciences of the United States of America·B LiuK Shuai
Jun 8, 2001·Annual Review of Biochemistry·A M NäärR Tjian
Oct 5, 2001·Molecular Cell·T KahyoH Yasuda
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Darja Schmidt, Stefan Müller
Jun 22, 2002·Molecular and Cellular Biology·Noora KotajaJorma J Palvimo
Dec 10, 2002·The Journal of Biological Chemistry·Edward E SchmidtMario R Capecchi
Jul 30, 2003·Molecular Biology and Evolution·Alla A Bondareva, Edward E Schmidt
Dec 20, 2003·Cellular and Molecular Life Sciences : CMLS·D Schmidt, S Müller
Jun 11, 2004·Annual Review of Biochemistry·Erica S Johnson
Jul 29, 2004·The Journal of Biological Chemistry·Gang Zheng, Yu-Chung Yang
Oct 29, 2004·Molecular and Cellular Biology·Marcin P KlejmanH T Marc Timmers
Dec 2, 2004·Molecular and Cellular Biology·Eliana MunarrizVincenzo De Laurenzi
Jan 18, 2005·Trends in Biochemical Sciences·H Th Marc Timmers, Làszlò Tora
Jan 20, 2005·Molecular and Cellular Biology·Bin LiuKe Shuai
Aug 2, 2005·Nature Reviews. Immunology·Ke Shuai, Bin Liu

❮ Previous
Next ❯

Citations

Nov 15, 2006·Molecular and Cellular Biology·Jun WangRobert J Schwartz
Feb 23, 2011·Molecular Cancer·Anne Hege Alm-KristiansenOdd S Gabrielsen
Jun 23, 2009·Biochimica Et Biophysica Acta·Justin R PriggeEdward E Schmidt
Mar 29, 2016·Basic Research in Cardiology·K E O'SullivanJ P Hurley
Dec 18, 2007·Molecular Immunology·Tanja IlmarinenIsmo Ulmanen
Dec 11, 2008·The Biochemical Journal·Hsiang-Tsan HsiehMeng-Chun Hu

❮ Previous
Next ❯

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.