Interaction of proteins in solution from small-angle scattering: a perturbative approach

Biophysical Journal
Francesco SpinozziFlavio Carsughi

Abstract

In this work an improved methodology for studying interactions of proteins in solution by small-angle scattering is presented. Unlike the most common approach, where the protein-protein correlation functions g(ij)(r) are approximated by their zero-density limit (i.e., the Boltzmann factor), we propose a more accurate representation of g(ij)(r) that takes into account terms up to the first order in the density expansion of the mean-force potential. This improvement is expected to be particularly effective in the case of strong protein-protein interactions at intermediate concentrations. The method is applied to analyze small-angle x-ray scattering data obtained as a function of the ionic strength (from 7 to 507 mM) from acidic solutions of beta-lactoglobulin at the fixed concentration of 10 gl(-1). The results are compared with those obtained using the zero-density approximation and show significant improvement, particularly in the more demanding case of low ionic strength.

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Citations

Dec 20, 2011·Interdisciplinary Sciences, Computational Life Sciences·Domenico Gazzillo, Achille Giacometti
Jul 3, 2009·Journal of the Royal Society, Interface·Maria Grazia OrtoreDaniela Russo
Jan 10, 2012·The Journal of Chemical Physics·Maria Grazia OrtoreFrancesco Spinozzi
Mar 28, 2008·European Biophysics Journal : EBJ·Raffaele SinibaldiPaolo Mariani
May 29, 2003·Biophysical Journal·Michael GottschalkBertil Halle
Jun 30, 2007·The Journal of Chemical Physics·Raffaele SinibaldiPaolo Mariani
May 21, 2005·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Achille GiacomettiTushar Kanti Das
Oct 15, 2008·Physical Review. E, Statistical, Nonlinear, and Soft Matter Physics·Domenico GazzilloAchille Giacometti
May 17, 2017·Biophysics Reviews·Francesco SpinozziMaria Grazia Ortore
Sep 25, 2008·The Journal of Physical Chemistry. B·Maria Grazia OrtorePaolo Mariani
May 1, 2021·Scientific Reports·Lucia SilvestriniFrancesco Spinozzi

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