PMID: 9546049Apr 18, 1998Paper

Interaction of quinones with Arabidopsis thaliana thioredoxin reductase

Biochimica Et Biophysica Acta
D BironaiteN K Cénas

Abstract

In view of the ubiquitous role of the thioredoxin/thioredoxin reductase (TRX/TR) system in living cells, the interaction of Arabidopsis thaliana NADPH-thioredoxin reductase (EC 1.6.4.5) with quinones, an important class of redox cycling and alkylating xenobiotics, was studied. The steady-state reactions of A. thaliana TR with thioredoxin (TRX) and reaction product NADP+ inhibition patterns were in agreement with a proposed model of E. coli enzyme (B.W. Lennon, C.H. Williams, Jr., Biochemistry, vol. 35 (1996), pp. 4704-4712), that involved enzyme cycling between four- and two-electron reduced forms with FAD being reduced. Quinone reduction by TR proceeded via a mixed single- and two-electron transfer, the percentage of single-electron flux being equal to 12-16%. Bimolecular rate constants of quinone reduction (kcat/km) and reaction catalytic constants (kcat) increased upon an increase in quinone single-electron reduction potential. E(1)7. In several cases, the kcat of quinone reduction exceeded kcat of TRX reduction, suggesting that quinones intercepted electron flux from TR to TRX. Incubation of reduced TR with alkylating quinones resulted in a rapid loss of TRX-reductase activity, while quinone reduction rate was unchanged. In...Continue Reading

References

Nov 1, 1992·European Journal of Biochemistry·M R FernandoS Minakami
Sep 1, 1992·Archives of Biochemistry and Biophysics·D A BironaitéS A Usanov
Apr 1, 1992·Biochemical Pharmacology·B L Mau, G Powis
Jan 1, 1991·Chemico-biological Interactions·P J O'Brien
Oct 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·J KuriyanG B Henderson
Apr 1, 1990·Cephalalgia : an International Journal of Headache·J D CarrollJ McAinsh
Jul 1, 1990·The Biochemical Journal·J VienozinskisJ Kulys
Apr 29, 1986·Biochemical and Biophysical Research Communications·K U Schallreuter, J M Wood
Jan 1, 1989·Free Radical Biology & Medicine·G Powis
Mar 23, 1989·Biochimica Et Biophysica Acta·N K CénasJ J Kulys
Jan 1, 1989·Journal of Steroid Biochemistry·M WiegandK Eisele
Aug 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·G B HendersonA Cerami
Mar 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·P L ChesisB N Ames
Nov 1, 1994·Archives of Biochemistry and Biophysics·B B BuchananR M Lozano
Dec 1, 1994·Archives of Biochemistry and Biophysics·N CénasK Ollinger
Mar 1, 1994·Proceedings of the National Academy of Sciences of the United States of America·H SchenkK Schulze-Osthoff
Feb 16, 2008·The Journal of Biological Chemistry·Madalena Martins Sant'ana BarrosoTatiana Coelho-Sampaio
Apr 8, 2011·Chemistry : a European Journal·Guo-Liang WangGuo-Xin Jin

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Citations

Aug 4, 2004·Cancer Chemotherapy and Pharmacology·Dashnamoorthy Ravi, Kumuda C Das
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·P. Schurmann, J.-P. Jacquot
Jan 18, 2006·Journal of Photochemistry and Photobiology. B, Biology·Nadezhda S Kudryasheva
Oct 15, 2009·Molecular Plant·Talaat BashandyJean-Philippe Reichheld
Nov 14, 1998·Biochimica Et Biophysica Acta·V MiskinieneN Cenas
Aug 26, 2014·Environmental Science and Pollution Research International·N S Kudryasheva, A S Tarasova
May 15, 2016·Free Radical Biology & Medicine·Natalia SasoniDiego G Arias
Nov 8, 2003·The Journal of Biological Chemistry·Narimantas CenasElias S J Arnér
Aug 28, 2021·International Journal of Molecular Sciences·Narimantas ČėnasLidija Kosychova

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