PMID: 38219Nov 1, 1978

Interaction of radical anion probes with glucoamylase I from Aspergillus niger

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
P L CopperJ V Davies

Abstract

The radical anions (SCN)2.- and Br2.- produced during a pulse radiolysis of the respective potassium salts have been used to study the tryptophan residues of the glucoenzyme, glucoamylase I (EC 3.2.1.3.). At neutral pH, Br2.- reacted with the tryptophan residues of glucoamylase I as expected from previous studies of proteins and free amino acids. However, (SCN)2.- at neutral and high pH was surprisingly unreactive towards the native enzyme. Reaction did occur, however, between (SCN)2.- and glucoamylase from which one-third of the covalently bound carbohydrate had been removed, producing a tryptophyl radical. Reaction also occured between (SCN)2.- and glucoamylase I inactivated by treatment with sodium dodecyl sulphate, but the tryptophan residues were not involved. It is concluded from the results that two 'types' of tryptophan residues are found in glucoamylase I; both are attacked by Br2.- but only one type is attacked by (SCN)2.-.

References

Nov 1, 1976·International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine·C V EadsforthE W Thomas
Jul 13, 1970·Biochemical and Biophysical Research Communications·J H PazurD L Simpson
Nov 1, 1969·Archives of Biochemistry and Biophysics·D R LinebackC Rasmussen
Feb 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·R Smith, C Tanford

Related Concepts

Anions
Aspergillus niger
Bromides
Enzyme Activation
Glucoamylase G2
Free Radicals
Glucosidase
Hydrogen-Ion Concentration
Thiocyanates
PMS-Tryptophan

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Computational Methods for Protein Structures

Computational methods employing machine learning algorithms are powerful tools that can be used to predict the effect of mutations on protein structure. This is important in neurodegenerative disorders, where some mutations can cause the formation of toxic protein aggregations. This feed follows the latests insights into the relationships between mutation and protein structure leading to better understanding of disease.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

Related Papers

International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
G E AdamsJ R Key
International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
F PochonJ Pucheault
International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine
J V DaviesS Mudher
© 2021 Meta ULC. All rights reserved