Interaction of radiolabeled antibodies with fungal cells and components of the immune system in vitro and during radioimmunotherapy for experimental fungal infection

The Journal of Infectious Diseases
Ekaterina DadachovaArturo Casadevall

Abstract

The usefulness of radioimmunotherapy (RIT) for infectious diseases was recently demonstrated for several fungal and bacterial infections, but the mechanisms by which RIT is effective against microbes are uncertain. We investigated the interaction between polysaccharide capsule-binding 18B7 monoclonal antibodies (MAbs) labeled with alpha-emitter 213Bi and Cryptococcus neoformans cells as well as between 213Bi-18B7 and components of immune system, both in vitro and in vivo. For 213Bi-18B7, the microbicidal effect was predominantly due to "direct-hit" killing, with some contribution from the "crossfire" effect. The efficacy of cell killing correlated with the binding capacity of the MAb to the capsule and was dependent on the MAb isotype. RIT also promoted the apoptosis-like death of fungal cells. Cooperation was observed in vitro between the antifungal activity of macrophages and RIT, suggesting the potential for synergistic action in vivo. RIT was associated with changes in concentration of the cytokines interleukin (IL)-2, IL-4, IL-10, tumor necrosis factor-alpha, and interferon-gamma, suggesting that the therapeutic effects of RIT may result from changes in the inflammatory response. The present results suggest that the antimi...Continue Reading

Citations

Jan 29, 2008·The Lancet Infectious Diseases·Antonio Cassone
Aug 21, 2010·Nature Reviews. Drug Discovery·Luis Ostrosky-ZeichnerJohn H Rex
Nov 10, 2007·Dalton Transactions : an International Journal of Inorganic Chemistry·Martin W Brechbiel
Aug 30, 2012·The Journal of Biological Chemistry·Alena JandaArturo Casadevall
Jul 3, 2010·The Journal of Infectious Diseases·Ruth A BryanEkaterina Dadachova
Oct 19, 2011·Antimicrobial Agents and Chemotherapy·Zewei JiangEkaterina Dadachova
Nov 23, 2006·Eukaryotic Cell·Michelle E MaxsonOscar Zaragoza
Feb 7, 2007·Infection and Immunity·Natasa MacuraArturo Casadevall
Jul 13, 2011·Interdisciplinary Perspectives on Infectious Diseases·Ekaterina Dadachova, Arturo Casadevall
Nov 9, 2006·PLoS Medicine·Ekaterina DadachovaHarris Goldstein
Nov 18, 2015·Immunological Investigations·Kausik Datta, Mawieh Hamad
Mar 25, 2015·Nuclear Medicine and Biology·Igor ShuryakEkaterina Dadachova
Jan 24, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·C Antachopoulos, T J Walsh
Aug 23, 2018·Cancer Biotherapy & Radiopharmaceuticals·Muath Helal, Ekaterina Dadachova
Jan 1, 2014·Antimicrobial Agents and Chemotherapy·Johanna RiveraArturo Casadevall
Apr 19, 2018·Biomédica : revista del Instituto Nacional de Salud·María Claudia CamposGerardo Moreno
Aug 28, 2009·Expert Opinion on Pharmacotherapy·Michele Ritter, David L Goldman
Oct 23, 2013·Immunology and Cell Biology·Marta CaridadeRuy M Ribeiro
Sep 6, 2014·Nuclear Medicine Communications·Alexander H StaudacherMichael P Brown
Oct 4, 2017·Current Medicinal Chemistry·Mateusz KowalikBarbara Barszcz
Mar 3, 2020·Frontiers in Microbiology·Muath HelalEkaterina Dadachova
Aug 27, 2014·Expert Review of Anticancer Therapy·Ruth A BryanEkaterina Dadachova
Feb 26, 2019·Frontiers in Pharmacology·Tomas Di MambroEmanuele Marra
Sep 19, 2007·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ekaterina DadachovaArturo Casadevall
Mar 19, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ekaterina RevskayaEkaterina Dadachova
Jun 3, 2021·Journal of Fungi·Alfred O AnkrahAndor W J M Glaudemans
Aug 14, 2021·Frontiers in Medicine·Jean-Pierre Pouget, Julie Constanzo

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Ekaterina DadachovaArturo Casadevall
Antimicrobial Agents and Chemotherapy
L R MartinezEkaterina Dadachova
Nature Reviews. Drug Discovery
Diane MilenicMartin W Brechbiel
© 2022 Meta ULC. All rights reserved