Interaction of recombinant human bone morphogenetic protein-2 with poly(d,l lactide-co-glycolide) microspheres

Pharmaceutical Development and Technology
S S DuggiralaP P Deluca

Abstract

The combination of recombinant human bone morphogenetic protein-2 (rhBMP-2) with poly(d,l lactide-co-glycolide) (PLGA) porous microspheres provided for "sustained release" of the protein from the microspheres. Soaking 50:50 PLGA microspheres in a buffered rhBMP-2 solution for a sufficient period of time to permit equilibrium binding enabled quantification of "free" and "bound" protein. "Free" protein is defined as protein present within the porous matrix of the microspheres, whereas "bound" refers to protein adsorbed to PLGA surfaces. Kinetics of the rhBMP-2-microsphere association revealed that equilibrium was attained within 8 hr for two buffer systems (arginine/histidine, pH 6.50; and glutamic acid/sodium glutamate, pH 4.50). Increasing the concentration of the rhBMP-2 stock solution used for the interaction studies from 0.025 to 1.0 mg/ml increased the amount of rhBMP-2 adsorbed and the concentration of free rhBMP-2. Beyond a 1.0 mg/mL concentration, only free rhBMP-2 levels increased. Linearized Langmuir treatment of the adsorption data yielded values corresponding to monolayer coverage of the microspheres (Cm) and the equilibrium adsorption constant (K) of 0.17 microgram/cm2 and 7.57 ml/mg, respectively. Studies performed...Continue Reading

References

Mar 1, 1992·Trends in Genetics : TIG·V Rosen, R S Thies
Jan 1, 1974·Journal of Biomedical Materials Research·A S Hoffman
Oct 1, 1993·Pharmaceutical Research·R A KenleyJ O Hollinger

❮ Previous
Next ❯

Citations

Jan 20, 2004·AAPS PharmSciTech·J A SchrierP P DeLuca
Jan 7, 1999·Drug Development and Industrial Pharmacy·R JainC T Rhodes
Mar 22, 2012·Advanced Drug Delivery Reviews·William J King, Paul H Krebsbach
Feb 24, 2009·Biomaterials·Diederik H R KempenWouter J A Dhert
Dec 17, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Xiaoqin WangDavid L Kaplan
Jun 17, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Diederik H R KempenMichael J Yaszemski
Jun 9, 2012·Journal of Tissue Engineering and Regenerative Medicine·Cheryl V RahmanErella Livne
Feb 18, 2015·Journal of Biomedical Materials Research. Part a·Farzana SharminYusuf Khan
Dec 1, 1999·Pharmaceutical Development and Technology·J A Schrier, P P DeLuca
Oct 9, 2008·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Abid Riaz Ahmed, Roland Bodmeier
Jun 26, 1998·Journal of Biomedical Materials Research·D A PuleoP P DeLuca
Nov 7, 1999·Biotechnology and Bioengineering·H UludagJ M Wozney
Mar 4, 2010·Journal of Biomedical Materials Research. Part a·Emily K CushnieCato T Laurencin
Jul 1, 1996·Pharmaceutical Development and Technology·S S DuggiralaP P DeLuca

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.