Interaction of RNA polymerase II and the small RNA machinery affects heterochromatic silencing in Drosophila.

Epigenetics & Chromatin
Harsh H Kavi, James A Birchler

Abstract

Heterochromatin is the tightly packaged dynamic region of the eukaryotic chromosome that plays a vital role in cellular processes such as mitosis and meiotic recombination. Recent experiments in Schizosaccharomyces pombe have revealed the structure of centromeric heterochromatin is affected in RNAi pathway mutants. It has also been shown in fission yeast that the heterochromatin barrier is traversed by RNA Pol II and that the passage of RNA Pol II through heterochromatin is important for heterochromatin structure. Thus, an intricate interaction between the RNAi machinery and RNA Pol II affects heterochromatin structure. However, the role of the RNAi machinery and RNA Pol II on the metazoan heterochromatin landscape is not known. This study analyses the interaction of the small RNA machinery and RNA Pol II on Drosophila heterochromatin structure. The results in this paper show genetic and biochemical interaction between RNA Pol II (largest and second largest subunit) and small RNA silencing machinery components (dcr-2, ago1, ago2, piwi, Lip [D], aub and hls). Immunofluorescence analysis of polytene chromosomes from trans-heterozygotes of RNA Pol II and different mutations of the small RNA pathways show decreased H3K9me2 and misl...Continue Reading

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Citations

Sep 6, 2011·Nature Structural & Molecular Biology·Francisca E Reyes-TurcuShiv I S Grewal
Nov 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Mikhail S KlenovVladimir A Gvozdev
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Methods Mentioned

BETA
transgenic
immunoprecipitation
ChIP
co-immunoprecipitation
co-IP
pull down
pulldown
fluorescence microscopy

Software Mentioned

Photoshop
Image gauge ( NIH )

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