Interaction of Salmonella phage P22 with its O-antigen receptor studied by X-ray crystallography.

Biological Chemistry
S SteinbacherR Seckler

Abstract

The O-antigenic repeating units of the Salmonella cell surface lipopolysaccharides (serotypes A, B and D1) serve as receptors for phage P22. This initial binding step is mediated by the tailspike protein (TSP), which is present in six copies on the base plate of the phage. In addition to the binding activity, TSP also displays a low endoglycolytic activity, cleaving the alpha(1,3)-O-glycosidic bond between rhamnose and galactose of the O-antigenic repeats. The crystal structure of TSP in complex with receptor fragments allowed to identify the receptor binding site for the octasaccharide product of the enzymatic action of TSP on delipidated LPS and the active site consisting of Asp392, Asp395 and Glu359. The structure comprises a large right-handed parallel beta-helix of 13 turns. These fold independently in the trimer, whereas the N-terminus forms a cap-like structure and the C-terminal parts of the three polypeptide strands merge to a single common domain. In addition, TSP has served as model system for the folding of large, multisubunit proteins. Its folding pathway is influenced by a large number of point mutations, classified as lethal, temperature sensitive or general suppressor mutations, which influence the partitioning ...Continue Reading

References

Feb 1, 1977·The Journal of General Virology·U Eriksson, A A Lindberg
Jun 1, 1979·The Journal of General Virology·U ErikssonA A Lindberg
Jul 2, 1991·Biochemistry·A FuchsR Seckler
Jan 1, 1989·Proteins·G M Air, W G Laver
Jun 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·D Goldenberg, J King
Apr 1, 1980·Journal of Virology·P B Berget, A R Poteete
Dec 1, 1994·Current Opinion in Structural Biology·J D McCarter, S G Withers
Mar 1, 1995·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·M D Yoder, F Jurnak
Sep 15, 1995·Structure·G Davies, B Henrissat
Jan 1, 1996·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·J KingA Mitraki
Apr 1, 1996·Current Opinion in Structural Biology·L Liljas
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·S SteinbacherR Huber
Feb 1, 1967·Proceedings of the National Academy of Sciences of the United States of America·J V IsraelM Levine

❮ Previous
Next ❯

Citations

Aug 13, 2005·Archives of Virology·R VillafaneC Salgado
Mar 27, 2001·Protein Science : a Publication of the Protein Society·C Haase-PettingellJ King
Oct 29, 2000·Journal of Bacteriology·C Vander Byl, A M Kropinski
Jul 3, 2003·Journal of Bacteriology·Peter R WeigeleJonathan King
Nov 23, 2006·Gene·Milka Zayas, Robert Villafane
Sep 23, 2014·International Journal of Food Microbiology·Paola ZinnoGianluigi Mauriello
Jan 1, 2010·Annual Review of Food Science and Technology·Brid CoffeyR Paul Ross
Nov 5, 2013·Applied and Environmental Microbiology·Hakdong ShinSangryeol Ryu
Nov 26, 2013·Applied and Environmental Microbiology·Minsik KimSangryeol Ryu
Jun 23, 2012·The Analyst·Amit SinghStephane Evoy
Nov 22, 2013·Biochemical Society Transactions·Rogier Louwen, Peter van Baarlen
Mar 25, 2017·Applied Microbiology and Biotechnology·Agnieszka LatkaZuzanna Drulis-Kawa
Apr 23, 2008·Current Protocols in Protein Science·James M BenevidesGeorge J Thomas
Aug 24, 2001·Journal of Molecular Biology·A J ClarkT S Dhillon

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibody Specificity

Antibodies produced by B cells are highly specific for antigen as a result of random gene recombination and somatic hypermutation and affinity maturation. As the main effector of the humoral immune system, antibodies can neutralize foreign cells. Find the latest research on antibody specificity here.