Interaction of Sclerotinia sclerotiorum with Brassica napus: cloning and characterization of endo- and exo-polygalacturonases expressed during saprophytic and parasitic modes

Fungal Genetics and Biology : FG & B
Rugang LiDwayne D Hegedus

Abstract

Five major and several minor PG isoenzymes were identified in a Sclerotinia sclerotiorum isolate from Brassica napus by isoelectric focusing and pectin gel overlays. Using a combination of degenerate PCR and expressed sequence tags (ESTs) four endo-polygalacturonase (PG) genes, designated as sspg1d, sspg3, sspg5, and sspg6, and two exo-PG genes, ssxpg1 and ssxpg2, were identified. SSPG1d is a member of the PG gene family previously described by Fraissinet-Tachet et al. [Curr. Genet. 29 (1995) 96]. The mature SSPG1d is a neutral PG, whereas fully processed SSPG3, SSPG5, and SSPG6 are acidic enzymes. Under saprophytic growth conditions, sspg1d, sspg3, sspg5, and ssxpg1 expression was induced by pectin and galacturonic acid and subject to catabolite repression by glucose. Conditions could not be identified under which sspg6 or ssxpg2 were expressed well. Transfer of mycelia from liquid media to solid substrates induced expression of sspg1d suggesting that it may also be regulated by thigmotrophic interactions. Under pathogenic conditions, sspg1d was highly expressed during infection. sspg3 was also expressed during infection, albeit at lower levels than sspg1d, whereas sspg5, sspg6, and ssxpg1 were expressed only weakly.

References

Aug 15, 1992·European Journal of Biochemistry·H J BussinkJ Visser
Jul 1, 1996·Current Microbiology·L Fraissinet-Tachet, M Fevre
Oct 13, 1998·Molecular Plant-microbe Interactions : MPMI·A ten HaveJ A van Kan
Apr 2, 1999·Applied and Environmental Microbiology·J P WubbenJ Visser
Apr 8, 2000·Molecular Plant-microbe Interactions : MPMI·F I García-MaceiraM I Roncero
May 10, 2000·FEMS Microbiology Letters·F WagnerW Schäfer
Sep 7, 2000·Molecular Plant-microbe Interactions : MPMI·A L PowellJ M Labavitch
Jan 3, 2001·Applied and Environmental Microbiology·J A Rollins, M B Dickman
Apr 25, 2001·Applied and Environmental Microbiology·F I García-MaceiraM I Roncero
Jul 18, 2001·Fungal Genetics and Biology : FG & B·A ten HaveJ A van Kan
Nov 10, 2001·Annual Review of Phytopathology·S L Tucker, N J Talbot
Mar 15, 2002·The Journal of Biological Chemistry·Nathan C Rockwell, Robert S Fuller
Aug 9, 2002·FEMS Microbiology Letters·Pascale CottonMichel Fevre
May 1, 1991·Applied and Environmental Microbiology·C RiouM Fevre

❮ Previous
Next ❯

Citations

Nov 6, 2007·Molecular Plant-microbe Interactions : MPMI·Xiaomei Guo, Henrik U Stotz
Mar 7, 2008·Molecular Plant-microbe Interactions : MPMI·Chih-Hang WuRuey-Fen Liou
Jun 20, 2012·BMC Plant Biology·Corina M FusariNorma B Paniego
Jan 19, 2010·Molecular Plant Pathology·Zafer Dallal BashiMohammed H Borhan
Feb 7, 2006·Phytochemistry·Adele Di MatteoFelice Cervone
Jan 1, 2006·Molecular Plant Pathology·Melvin D BoltonBerlin D Nelson
Aug 23, 2005·FEMS Microbiology Letters·Dwayne D Hegedus, S Roger Rimmer
Jul 2, 2016·Molecular Plant-microbe Interactions : MPMI·Silvio TundoRenato D'Ovidio
Mar 31, 2017·BMC Genomics·Shirin SeifbarghiDwayne D Hegedus
Jun 30, 2018·Annual Review of Phytopathology·Liangsheng XuWeidong Chen
Jul 27, 2018·Phytopathology·Xiaofei Liang, Jeffrey A Rollins
Jan 30, 2019·Applied Microbiology and Biotechnology·Kevin SchmitzJ Philipp Benz
Sep 26, 2020·Frontiers in Plant Science·Silvia Rodríguez-PiresEduardo A Espeso

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aspergillosis

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.

Aspergillosis (ASM)

Aspergillosis is the name given to a wide variety of diseases caused by infection by fungi of the genus Aspergillus. Aspergillosis occurs in chronic or acute forms which are clinically very distinct. Most cases of acute aspergillosis occur in patients with severely compromised immune systems. Chronic colonization or infection can cause complications in people with underlying respiratory illnesses. Discover the latest research on aspergillosis here.