Interaction of singlet oxygen with 5'-nucleotidase in rat hearts

Journal of Molecular and Cellular Cardiology
X ZhaiM Ashraf

Abstract

This study was aimed to determine whether singlet oxygen (1O2) attenuates 5'-nucleotidase activity in the ischemic myocardium. Isolated rat hearts were exposed to either exogenous 1O2 produced by irradiating rose bengal or 40-min ischemia and reperfusion. Ecto-5'-nucleotidase activity was inhibited by exogenous 1O2 (3.74 +/- 0.38 mumol/min/g dry weight), when compared with normal control (7.52 +/- 0.41 mumol/min/g dry weight; P < 0.05). The enzymatic activity was significantly preserved by histidine (25 mM)--a 1O2 scavenger (7.04 +/- 0.61 mumol/min/g dry weight; P < 0.05 v rose bengal group). After ischemia, the activity of ecto-5'-nucleotidase was greatly reduced (2.51 +/- 0.25 mumol/min/g dry weight), when compared with normal control. Histidine significantly enhanced ecto-5'-nucleotidase activity (6.55 +/- 0.52 mumol/min/g dry weight, P < 0.05 v ischemic control). Adenosine release was consistent with ecto-5'-nucleotidase activity. The time course studies of effects of 1O2 on coronary flow, cardiac function, and LDH release revealed that the damage by 1O2 to ecto-5'-nucleotidase activity and adenosine release primarily accounted for impaired coronary flow, cardiac dysfunction, and impaired cardiac metabolism. Lipid peroxidat...Continue Reading

Citations

Jul 4, 2012·Purinergic Signalling·Viviane do Carmo Gonçalves SouzaDaniela B R Leal
Apr 9, 2001·Antioxidants & Redox Signaling·M C ToufektsianJ G de Leiris
Dec 22, 2011·Biochemical and Biophysical Research Communications·Hiroshi YoshiiYasuhisa Fujibayashi
May 21, 1999·The Australian and New Zealand Journal of Psychiatry·B Y Ng

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