Interaction of streptococcal cell wall components with fibrinogen. I. adsorption of fibrinogen by immobilized T-proteins of streptococcus pyogenes

Immunobiology
K H Schmidt, W Köhler

Abstract

Immobilized streptococcal T1-, T3- and T4-proteins to AH-Sepharose 4B were tested for their ability to absorb human fibrinogen. Purified fibrinogen and plasma samples were used for affinity chromatography. T1-protein was able to retain specifically fibrinogen from plasma. T4-protein bound fibrinogen in a similar manner, but it was not as specific as T1-protein. T3-protein failed to bind purified fibrinogen as well as fibrinogen from plasma. Adsorption of fibrinogen was accomplished using a 0.05 M phosphate/0.2 M NaCl/0.02% NaN3/pH 7.0 buffer system followed by elution with 0.05 M PO4/1 M NaCl/0.02% NaN3 to remove non specifically bound components. Retained components were eluted with 8 M Urea/0.025 M NaOAc, pH 5 and the fractions analyzed for fibrinogen content by SDS polyacrylamide gel electrophoresis. The presence of fibrinogen was determined by observations of the characteristic A alpha, B beta and gamma chain bands.

References

Jan 1, 1978·Immunological Reviews·S G Johansson, M Inganäs
Jan 1, 1974·Scandinavian Journal of Immunology·M Harboe, I Fölling

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Citations

Jan 1, 1983·Medical Microbiology and Immunology·C LämmlerH Blobel
Dec 14, 1999·The Journal of Laboratory and Clinical Medicine·J L Coleman, J L Benach
Apr 1, 1987·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·K H SchmidtW Köhler
Jun 1, 1987·Zentralblatt Für Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology·J HavlícekH Havlícková
Apr 1, 1985·Acta Pathologica, Microbiologica, Et Immunologica Scandinavica. Section B, Microbiology·A ReuterswärdG Kronvall
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Aug 1, 1985·Journal of Clinical Microbiology·L J ForresterJ T Barrett
Feb 1, 1983·Infection and Immunity·G E KurpiewskiJ T Barrett

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