Interaction of syntaxin with alpha-fodrin, a major component of the submembranous cytoskeleton

Biochemical and Biophysical Research Communications
M NakanoHiromichi Shirataki

Abstract

The soluble N-ethyl maleimide-sensitive factor attachment protein receptor machinery is involved in membrane docking and fusion. In this machinery, the syntaxin family is a central coordinator and participates in multiple protein-protein interactions. In this study we have shown that alpha-fodrin, nonerythroid spectrin, is a new binding partner of the syntaxin family. alpha-Fodrin bound to syntaxin-1a, -3, and -4, all of which are localized on the plasma membrane. Syntaxin-3 interacted with alpha-fodrin in dose-dependent and saturable manners but not with alpha-spectrin, erythroid spectrin. Syntaxin-3 interacted with alpha-fodrin through its C-terminal coiled-coil region. Binding of Munc18 or SNAP-25 to syntaxin-1a inhibited the interaction of alpha-fodrin with syntaxin-1a. Available evidence indicates that alpha-fodrin is implicated in exocytosis, but a precise mode of action of alpha-fodrin in exocytosis remains unclear. Our results suggest that alpha-fodrin regulates exocytosis through the interaction with members of the syntaxin family.

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Citations

Aug 29, 2003·Biophysical Journal·Aleksandar JeremicBhanu P Jena
Aug 24, 2013·Experimental & Molecular Medicine·Michael A Kalwat, Debbie C Thurmond
Feb 1, 2003·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Satoru NogamiHiromichi Shirataki
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Nov 19, 2003·Biochemical and Biophysical Research Communications·Satoru NogamiHiromichi Shirataki
Jun 5, 2012·Journal of Proteomics·Jin-Sook LeeBhanu P Jena
Jun 9, 2004·Biochemical and Biophysical Research Communications·Satoru NogamiHiromichi Shirataki
Aug 30, 2005·Annals of the New York Academy of Sciences·Torsten Witte
Apr 20, 2005·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Kenji YoshidaHiromichi Shirataki
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