Interaction of TAp73 and breast cancer-associated gene 3 enhances the sensitivity of cervical cancer cells in response to irradiation-induced apoptosis

Cancer Research
Thomas Ho-Yin Leung, Hextan Yuen-Sheung Ngan

Abstract

Identification of proteins that are involved in the sensitivity of radiotherapy of cancers is important to enhance the response to cancer treatment. Expression of TAp73 is associated with the sensitivity of radiotherapy in cervical cancer patients, suggesting it plays an important role in controlling radiosensitivity. Here, by using yeast two-hybrid system, we identify breast cancer-associated gene 3 (BCA3) as the first and novel protein interacting partner of TAp73. By coimmunoprecipitation and Western blot analysis, we confirm that TAp73 binds with and stabilizes BCA3 in cervical cancer cell line HeLa. Immunofluorescence staining indicates that BCA3 is localized in the cytoplasm and nucleus. Interestingly, when coexpressed with TAp73, BCA3 interacts and colocalizes with TAp73 at the mitochondria. Mutagenesis reveals that the oligomerization domain of TAp73 is responsible for the interaction with BCA3. Furthermore, BCA3 augments the transactivation activity of TAp73 on bax promoter and protein expression. In addition, the expression of BCA3 also increases the sensitivity of TAp73-transfected cells in response to gamma-irradiation-induced apoptosis. Western blot analysis also shows that TAp73 and BCA3 induce activation of caspa...Continue Reading

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Citations

Jan 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Mira SastriSusan S Taylor
Oct 31, 2013·International Journal of Molecular Sciences·Hongjuan YuHerman H W Silljé
Jun 3, 2014·Retrovirology·Michaela RumlováTomáš Ruml
Aug 6, 2013·Genes, Chromosomes & Cancer·Beatriz SoldevillaGemma Domínguez
Mar 25, 2014·The Journal of General Virology·Michaela RumlováTomáš Ruml
May 28, 2013·Cell Cycle·Cuixia DiZhongtian Bai
Apr 21, 2018·Viruses·Michaela RumlováTomáš Ruml
Jul 25, 2013·British Journal of Cancer·T H-Y LeungH Y-S Ngan
Sep 17, 2017·Oncotarget·Olivier BillantCécile Voisset

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