Interaction of Tet repressor with operator DNA and with tetracycline studied by infrared and Raman spectroscopy

Biophysical Journal
Christoph KrafftH Welfle

Abstract

Tet repressor (TetR) is involved in the most abundant mechanism of tetracycline (Tc) resistance of gram-negative bacteria. Raman spectra were measured for the class D TetR protein, for an oligodeoxyribonucleotide with sequence corresponding to operator site O1, and for the TetR:oligonucleotide complex. TetR forms a complex with [Ni-Tc]+, which does not bind to operator DNA. Raman and infrared measurements indicate nearly identical conformations of TetR with and without [Ni-Tc]+. Differences between the experimental spectrum of the TetR:operator DNA complex and the computed sum of the component spectra provide direct spectroscopic evidence for changes in DNA backbone torsions and base stacking, rearrangement of protein backbone, and specific contacts between TetR residues and DNA bases. Complex formation is connected with intensity decrease at 1376 cm(-1) (participation of thymine methyl groups), intensity increase at 1467 cm(-1) (hydrogen bond formation at guanine N7), decreased intensity ratio I854/I823 (increased hydrophobicity of tyrosine environment), increased intensity at 1363 cm(-1) (increased hydrophobicity of tryptophan ring environment), differences in the range 670-833 cm(-1) (changes in B-DNA backbone torsions and b...Continue Reading

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Citations

Nov 19, 2003·European Journal of Biochemistry·Sylwia Kedracka-Krok, Zygmunt Wasylewski
Jun 6, 2000·Biological Chemistry·K WeiszH Welfle
May 9, 2014·Spectrochimica Acta. Part A, Molecular and Biomolecular Spectroscopy·Deepak K Jangir, Ranjana Mehrotra
Dec 11, 2008·Biotechnology Letters·Noriko KarasakiTakahiro Kusakabe
Jun 13, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Gottfried J PalmWinfried Hinrichs
Oct 17, 2017·Environmental Science and Pollution Research International·Yanlong SunPeng Wang
Jun 2, 2018·Environmental Science and Pollution Research International·Yanlong SunPeng Wang
Jun 27, 2019·International Journal of Molecular Sciences·Sara SignorelliAnna Rita Bizzarri
Feb 10, 2009·Analytical Chemistry·Benjamin Moody, Gregory McCarty
Jul 17, 1999·Analytical Chemistry·C K LariveS Bogdanowich-Knipp

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