Interaction of the 33 kDa extrinsic protein with photosystem II: rebinding of the 33 kDa extrinsic protein to photosystem II membranes which contain four, two, or zero manganese per photosystem II reaction center

Biochemistry
C Leuschner, Terry M Bricker

Abstract

The 33 kDa extrinsic protein of photosystem II acts to enhance oxygen evolution and to stabilize the manganese cluster at low chloride concentrations. Due to controversies concerning the stoichiometry of this protein [Miyao, M., & Murata, N. (1989) Biochim. Biophys. Acta 977, 315-321, versus Xu, Q., & Bricker, T. M. (1992) J. Biol. Chem. 267. 25816-25821] we have examined the rebinding of this protein to PS II membrane preparations which contain four, two, or zero manganese per photosystem II reaction center. After rebinding, immunoquantification of the 33 kDa extrinsic protein demonstrated that each of these photosystem II membrane preparations strongly bound two copies of the 33 kDa extrinsic protein per photosystem II reaction center. The first and second stoichiometric binding constants (Ka1 and Ka2) for the binding of the 33 kDa protein to PS II centers containing four manganese were 0.42 and 0.67 nM(-1), respectively. Disruption of the manganese cluster either by removal of the chloride-sensitive manganese or extraction of the manganese cluster by alkaline Tris led to a 5-6-fold decrease in Ka1 and about a 3-fold decrease in Ka2. In all cases the binding of the two copies of the 33 kDa extrinsic protein exhibited positive...Continue Reading

References

Apr 1, 1990·Photosynthesis Research·T M Bricker

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Citations

Aug 22, 2008·Photosynthesis Research·Isao EnamiJian-Ren Shen
Apr 9, 2013·Photosynthesis Research·Terry M BrickerLaurie K Frankel
Oct 28, 2011·Proceedings of the National Academy of Sciences of the United States of America·Haijun LiuHimadri B Pakrasi
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Ben HankamerEgbert J. Boekema
May 4, 2006·Annual Review of Plant Biology·Nathan Nelson, Charles F Yocum
Jan 14, 2016·Planta·Johnna L RooseTerry M Bricker
Feb 15, 2011·Journal of Photochemistry and Photobiology. B, Biology·Hana Popelkova, Charles F Yocum
Aug 2, 2011·Biochimica Et Biophysica Acta·Terry M BrickerJulian J Eaton-Rye
Nov 12, 1996·Biochimica Et Biophysica Acta·A Seidler

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