Interaction of the Arabidopsis receptor protein kinase Wak1 with a glycine-rich protein, AtGRP-3

The Journal of Biological Chemistry
Ae Ran ParkOhkmae K Park

Abstract

The Arabidopsis wall-associated receptor kinase, Wak1, is a member of the Wak family (Wak1-5) that links the plasma membrane to the extracellular matrix. By the yeast two-hybrid screen, we found that a glycine-rich extracellular protein, AtGRP-3, binds to the extracellular domain of Wak1. Further in vitro binding studies indicated that AtGRP-3 is the only isoform among the six tested AtGRPs that specifically interacts with Waks, and the cysteine-rich carboxyl terminus of AtGRP-3 is essential for its binding to Wak1. We also show that Wak1 and AtGRP-3 form a complex with a molecular size of approximately 500 kDa in vivo in conjunction with the kinase-associated protein phosphatase, KAPP, that has been shown to interact with a number of plant receptor-like kinases. Binding of AtGRP-3 to Wak1 is shown to be crucial for the integrity of the complex. Wak1 and AtGRP-3 are both induced by salicylic acid treatment. Moreover, exogenously added AtGRP-3 up-regulates the expression of Wak1, AtGRP-3, and PR-1 (for pathogenesis-related) in protoplasts. Taken together, our data suggest that AtGRP-3 regulates Wak1 function through binding to the cell wall domain of Wak1 and that the interaction of Wak1 with AtGRP-3 occurs in a pathogenesis-rel...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·B D KohornT A Smith
May 1, 1990·The Plant Cell·D E de OliveiraJ Botterman
Apr 1, 1993·The Plant Journal : for Cell and Molecular Biology·D E de OliveiraM Van Montagu
Jan 1, 1993·The Plant Cell·A M Showalter
Aug 16, 1996·The Journal of Biological Chemistry·Z H HeB D Kohorn
Mar 15, 1997·The Biochemical Journal·P Wojtaszek
Jan 1, 1997·Annual Review of Cell and Developmental Biology·D J Cosgrove
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·Z H HeB D Kohorn
Nov 18, 1998·Current Opinion in Cell Biology·N Boudreau, M J Bissell
Dec 9, 1998·Proceedings of the National Academy of Sciences of the United States of America·B P ThommaW F Broekaert
Mar 6, 1999·Current Opinion in Plant Biology·K LeaseJ C Walker
Mar 6, 1999·Current Opinion in Plant Biology·R Pennell
Mar 11, 1999·The Plant Cell·H B Smith
Aug 14, 1999·Science·F G Giancotti, E Ruoslahti
Sep 11, 1999·Trends in Cell Biology·B D Kohorn
Oct 6, 1999·Current Opinion in Cell Biology·C Streuli
Jan 27, 2000·Cell·T Hunter
Jan 29, 2000·The Plant Journal : for Cell and Molecular Biology·C DomingoP Vera
Jun 20, 2000·Biochimica Et Biophysica Acta·G Sachetto-MartinsD E de Oliveira
Aug 1, 2000·Science·A E TrotochaudS E Clark
Oct 14, 2000·Annual Review of Cell and Developmental Biology·A G McCubbin, T Kao
Apr 1, 1993·Plant Physiology·B. Keller
Jun 1, 1997·Annual Review of Plant Physiology and Plant Molecular Biology·Chris Lamb, Richard A. Dixon

❮ Previous
Next ❯

Citations

Oct 1, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·D A LalliA G Abbott
Dec 14, 2007·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Chang-Jiang ZhaoGuo-Dong Lu
Apr 1, 2008·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Vydehi Kanneganti, Aditya K Gupta
Mar 14, 2002·Current Opinion in Cell Biology·J Mark CockThierry Gaude
May 22, 2003·Trends in Plant Science·Gabrielle TichtinskyThierry Gaude
Aug 17, 2002·Current Opinion in Plant Biology·Colin Brownlee
Sep 24, 2009·Journal of Experimental Botany·Klaas Bouwmeester, Francine Govers
Oct 14, 2003·Plant Physiology·Frantisek BaluskaDiedrik Menzel
May 29, 2010·Plant Physiology·Christoph Ringli
Apr 23, 2010·Plant Physiology·Blaire J Steinwand, Joseph J Kieber
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Philip W Becraft
Jul 27, 2002·Annual Review of Cell and Developmental Biology·Elizabeth M Lord, Scott D Russell
Feb 10, 2011·BMC Plant Biology·Jorge Mc MondegoUNKNOWN Brazilian Coffee Genome Project Consortium
Jan 1, 2007·The Arabidopsis Book·Huachun WangJohn C Walker
Sep 23, 2003·Proceedings of the National Academy of Sciences of the United States of America·Gui-In LeeSteven R Van Doren
Jul 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Sushma NaithaniJune B Nasrallah
Dec 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Steven VandenabeeleFrank Van Breusegem
May 16, 2003·Proceedings of the National Academy of Sciences of the United States of America·Diego WengierJorge Muschietti
Feb 10, 2009·Plant Molecular Biology·Francisca BlancoLoreto Holuigue
May 13, 2009·Planta·Amanda MangeonGilberto Sachetto-Martins
Jul 7, 2009·Annual Review of Plant Biology·David ChevalierJohn C Walker
Apr 12, 2014·Annals of Botany·Timo Engelsdorf, Thorsten Hamann
Oct 19, 2001·Molecular Plant-microbe Interactions : MPMI·G J RairdanT P Delaney
Mar 18, 2010·Yi chuan = Hereditas·Yan PanChuan-Ping Yang

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.