PMID: 9420248Jan 7, 1998Paper

Interaction of the bovine papillomavirus E6 protein with the clathrin adaptor complex AP-1

Journal of Virology
X TongP M Howley

Abstract

The E6 gene of the bovine papillomavirus type 1 (BPV-1) is expressed in fibropapillomas caused by BPV-1 and in tissue culture cells transformed by BPV-1. It encodes one of the two major oncoproteins of BPV-1. In this study, we demonstrate an interaction between the BPV-1 E6 protein and AP-1, the TGN (trans-Golgi network)-specific clathrin adaptor complex. AP-1 is a four-subunit protein complex required for clathrin-mediated cellular transport from the TGN. The AP-1/E6 interaction was observed in vitro and in cells. The E6 binding site on AP-1 was mapped to the N-terminal trunk domain of the gamma subunit. BPV-1 E6 preferentially associated with membrane-bound AP-1 in cells but not with free cytosolic AP-1. BPV-1 E6 was further shown to be recruited to isolated Golgi membranes and to copurify with clathrin-coated vesicles. The recruitment of BPV-1 E6 to Golgi membranes was AP-1 independent, but the E6 interaction with AP-1 was required for its association with clathrin-coated vesicles. Furthermore, AP-1 proteins could compete with BPV-1 E6 for binding to Golgi membranes, suggesting that the recruitment of BPV-1 E6 and AP-1 to Golgi membranes involves a common factor. Taken together, our results suggest that cytosolic BPV-1 E6 is...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·L Petti, D DiMaio
Apr 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·T KirchhausenA E Davis
May 1, 1989·Journal of Virology·K H VousdenD R Lowy
Feb 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·Y C YangP M Howley
Jan 1, 1986·Journal of Virology·J T SchillerD R Lowy
Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·J T SchillerD R Lowy
Dec 1, 1994·American Journal of Clinical Pathology·N ColemanM A Stanley
Aug 1, 1994·Current Opinion in Cell Biology·M S Robinson
May 12, 1994·Nature·S L Schmid, M R Jackson
Nov 1, 1993·The Journal of Cell Biology·L M TraubS Kornfeld
Feb 1, 1994·International Journal of Cancer. Journal International Du Cancer·N Coleman, M A Stanley
Oct 1, 1993·The Journal of Cell Biology·M S Robinson
Aug 1, 1996·Current Opinion in Immunology·I H Frazer
Apr 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·X Tong, P M Howley
Oct 1, 1992·Trends in Cell Biology·M S Robinson

❮ Previous
Next ❯

Citations

Jul 10, 1999·Immunological Reviews·F M Brodsky
Jun 14, 2000·Journal of Virology·H A PancioL Ratner
Feb 24, 2001·Traffic·M Marsh, A Pelchen-Matthews
Nov 1, 2001·Annual Review of Cell and Developmental Biology·F M BrodskyD E Wakeham
May 16, 2008·Veterinary Research·Giuseppe Borzacchiello, Franco Roperto
Feb 21, 2007·International Journal of Gynecological Cancer : Official Journal of the International Gynecological Cancer Society·G Vazquez-OrtizM Salcedo
May 16, 2000·The Journal of Biological Chemistry·Q GaoV Band
Aug 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·Joseph DeMasiPeter M Howley
Apr 29, 2015·PloS One·Daniel Pérez-NúñezRicardo Madrid
Jul 10, 1999·Immunological Reviews·F M BrodskyE M Bennett
May 25, 2004·The Journal of Biological Chemistry·Jonathan C Trinidad, Jonathan B Cohen
Jun 5, 1998·The Journal of Biological Chemistry·J J ChenE J Androphy
Jun 3, 2000·Annual Review of Immunology·D TortorellaH L Ploegh
Oct 18, 2008·Veterinary Dermatology·Lubna Nasir, M Saveria Campo
Oct 11, 2005·Proceedings of the National Academy of Sciences of the United States of America·Yoshihiro NakataniAnna M Herlitz
Jun 29, 2007·Journal of Virology·Joseph DeMasiPeter M Howley
Jan 20, 2016·BioMed Research International·Rodrigo Pinheiro AraldiRita de Cassia Stocco
Apr 15, 2003·The Journal of General Virology·G ChambersL Nasir
Sep 20, 2019·Viruses·Axel KargerMaria Montoya
Feb 18, 2017·Genetics and Molecular Biology·Rodrigo Pinheiro AraldiWilly Beçak
Sep 3, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·R HeilkerP Crottet
Nov 2, 1999·Virology·P J Duerksen-HughesS B Schwartz
Sep 18, 1998·Biochimica Et Biophysica Acta·L Rapp, J J Chen
Aug 15, 1998·Trends in Cell Biology·J Oldridge, M Marsh
Aug 30, 2002·Biochemical and Biophysical Research Communications·Minjie DuJason J Chen
May 2, 1998·Science·H L Ploegh
Aug 19, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Rodrigo Pinheiro AraldiEdislane Barreiros de Souza

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.