The 18 kDa histone H1-like protein from Chlamydia trachomatis (Hc1) is a DNA-binding protein thought to be involved in condensation of the chlamydial chromosome during late stages in the chlamydial life cycle. Expression of Hc1 in Escherichia coli results in an overall relaxation of DNA and severely affects DNA, RNA and protein synthesis. We have analysed the interaction of Hc1 with single-stranded DNA and RNA by Southwestern and Northwestern blotting. Furthermore, we show that purified, recombinant Hc1 dramatically affects transcription and translation in vitro at physiologically relevant concentrations. These results were found to coincide with the formation of condensed Hc1-DNA and Hc1-RNA complexes as revealed by agarose gel electrophoresis and electron microscopy. The implications of these results for possible functions of Hc1 in vivo are discussed.
Ultrastructural studies of the nucleoids of the pleomorphic forms of Chlamydia psittaci 6BC: a comparison with bacteria
Complete nucleotide sequence of bacteriophage MS2 RNA: primary and secondary structure of the replicase gene
Lethal overproduction of the Escherichia coli nucleoid protein H-NS: ultramicroscopic and molecular autopsy
Expression and mutational analysis of the nucleoid-associated protein H-NS of Salmonella typhimurium
Identification and nucleotide sequence of a developmentally regulated gene encoding a eukaryotic histone H1-like protein from Chlamydia trachomatis
Immunocytochemical analysis of AlgP (Hp1), a histonelike element participating in control of mucoidy in Pseudomonas aeruginosa
Mucoid Pseudomonas aeruginosa in cystic fibrosis: signal transduction and histone-like elements in the regulation of bacterial virulence
AlgR3, a protein resembling eukaryotic histone H1, regulates alginate synthesis in Pseudomonas aeruginosa
Protein H1: a role for chromatin structure in the regulation of bacterial gene expression and virulence?
Characterization and identification of early proteins in Chlamydia trachomatis serovar L2 by two-dimensional gel electrophoresis.
Biosynthesis and disulfide cross-linking of outer membrane components during the growth cycle of Chlamydia trachomatis.
Synthesis of disulfide-bonded outer membrane proteins during the developmental cycle of Chlamydia psittaci and Chlamydia trachomatis
Preparation and chemical composition of the cell walls of mature infectious dense forms of meningopneumonitis organisms.
H1a, an E. coli DNA-binding protein which accumulates in stationary phase, strongly compacts DNA in vitro
Chlamydia trachomatis Mip-like protein has peptidyl-prolyl cis/trans isomerase activity that is inhibited by FK506 and rapamycin and is implicated in initiation of chlamydial infection
Molecular cloning and expression of hctB encoding a strain-variant chlamydial histone-like protein with DNA-binding activity
Synthesis of the Escherichia coli K-12 nucleoid-associated DNA-binding protein H-NS is subjected to growth-phase control and autoregulation
Characterization of integration host factor (IHF) binding upstream of the cysteine-rich protein operon (omcAB) promoter of Chlamydia trachomatis LGV serovar L2
Regulation of the Chlamydia trachomatis histone H1-like protein Hc2 is IspE dependent and IhtA independent
In silico prediction and functional validation of sigma28-regulated genes in Chlamydia and Escherichia coli
Translation inhibition of the developmental cycle protein HctA by the small RNA IhtA is conserved across Chlamydia
Chlamydial histone-DNA interactions are disrupted by a metabolite in the methylerythritol phosphate pathway of isoprenoid biosynthesis
Chlamydial metabolism revisited: interspecies metabolic variability and developmental stage-specific physiologic activities
Metabolic features of Protochlamydia amoebophila elementary bodies--a link between activity and infectivity in Chlamydiae
Identification of the base-pairing requirements for repression of hctA translation by the small RNA IhtA leads to the discovery of a new mRNA target in Chlamydia trachomatis
Emancipating Chlamydia: Advances in the Genetic Manipulation of a Recalcitrant Intracellular Pathogen
Purification of recombinant Chlamydia trachomatis histone H1-like protein Hc2, and comparative functional analysis of Hc2 and Hc1
Proteolytic clipping of histone tails: the emerging role of histone proteases in regulation of various biological processes
The repeated 36 amino acid motif of Chlamydia trachomatis Hc2 protein binds to the major groove of DNA
Characterization of a Chlamydia psittaci DNA binding protein (EUO) synthesized during the early and middle phases of the developmental cycle
The 18-kilodalton Chlamydia trachomatis histone H1-like protein (Hc1) contains a potential N-terminal dimerization site and a C-terminal nucleic acid-binding domain
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