Interaction of the disintegrin and cysteine-rich domains of ADAM12 with integrin alpha7beta1

Experimental Cell Research
Zhefeng ZhaoAnna Zolkiewska

Abstract

We describe a novel interaction between the disintegrin and cysteine-rich (DC) domains of ADAM12 and the integrin alpha7beta1. Integrin alpha7beta1 extracted from human embryonic kidney 293 cells transfected with alpha7 cDNA was retained on an affinity column containing immobilized DC domain of ADAM12. 293 cells stably transfected with alpha7 cDNA adhered to DC-coated wells, and this adhesion was partially inhibited by 6A11 integrin alpha7 function-blocking antibody. The X1 and the X2 extracellular splice variants of integrin alpha7 supported equally well adhesion to the DC protein. Integrin alpha7beta1-mediated cell adhesion to DC had different requirements for Mn2+ than adhesion to laminin. Furthermore, integrin alpha7beta1-mediated cell adhesion to laminin, but not to DC, resulted in efficient cell spreading and phosphorylation of focal adhesion kinase (FAK) at Tyr397. We also show that adhesion of L6 myoblasts to DC is mediated in part by the endogenous integrin alpha7beta1 expressed in these cells. Since integrin alpha7 plays an important role in muscle cell growth, stability, and survival, and since ADAM12 has been implicated in muscle development and regeneration, we postulate that the interaction between ADAM12 and inte...Continue Reading

References

Oct 19, 1995·Nature·T Yagami-HiromasaA Fujisawa-Sehara
Jan 1, 1995·Methods in Enzymology·J B Bjarnason, J W Fox
Aug 25, 1997·Experimental Cell Research·S CrawleyS J Kaufman
Nov 14, 1997·Nature Genetics·U MayerK von der Mark
Feb 7, 1998·The Journal of Biological Chemistry·B J GilpinU M Wewer
May 20, 1998·Nature Genetics·Y K HayashiK Arahata
Apr 13, 1999·Cell and Tissue Research·D J Burkin, S J Kaufman
May 18, 1999·The American Journal of Pathology·K IbaU M Wewer
Jun 13, 2000·The Journal of Biological Chemistry·Z ShiL J Murphy
Nov 30, 2000·Biochemical and Biophysical Research Communications·F LoechelU M Wewer
Dec 29, 2000·Journal of Muscle Research and Cell Motility·A BornemanA Fujisawa-Sehara
Jul 6, 2001·The Biochemical Journal·Y CaoA Zolkiewska
Jul 20, 2001·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J P Evans
Jul 31, 2001·The Journal of Biological Chemistry·S LiuM H Ginsberg
Nov 29, 2001·The Journal of Biological Chemistry·Lance C BridgesRon D Bowditch
Dec 18, 2001·The Journal of Biological Chemistry·Helga von der MarkErnst Pöschl
Apr 23, 2002·The Journal of Biological Chemistry·Alban GaultierDominique Alfandari
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Motomu ShimaokaTimothy A Springer
May 10, 2002·The American Journal of Pathology·Nobuko KawaguchiUlla M Wewer
Nov 5, 2002·The American Journal of Pathology·Pauliina KronqvistUlla M Wewer
Dec 17, 2002·Molecular and Cellular Biology·Tomohiro KurisakiAtsuko Sehara-Fujisawa
Jan 2, 2003·The Journal of Biological Chemistry·Charles Kumar ThodetiUlla M Wewer
Jan 7, 2003·Genes & Development·Darren F Seals, Sara A Courtneidge
Oct 1, 2003·Experimental Cell Research·Monika TomczukJudith M White
Oct 2, 2003·Current Opinion in Cell Biology·Judith M White

❮ Previous
Next ❯

Citations

Dec 3, 2004·Molecular Biology of the Cell·Peggy LafusteFrançois-Jérôme Authier
Aug 31, 2011·Molecular Cancer Research : MCR·Camilla FröhlichUlla M Wewer
Sep 30, 2015·Cell Adhesion & Migration·Julian K Christians, Alexander G Beristain
Feb 19, 2011·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·M KokozidouT Pufe
Nov 19, 2005·Scandinavian Journal of Medicine & Science in Sports·M D GroundsJ White
May 15, 2013·PloS One·Marta PrzewoźniakEdyta Brzoska
Jul 11, 2006·Progress in Neurobiology·Peng YangTheo Hagg
Sep 27, 2008·Tissue Engineering. Part B, Reviews·Kristel J M Boonen, Mark J Post
Feb 5, 2008·International Journal of Cancer. Journal International Du Cancer·Emilia DyczynskaAnna Zolkiewska
Mar 15, 2006·Differentiation; Research in Biological Diversity·Edyta BrzóskaJerzy Moraczewski
Feb 25, 2006·Micron : the International Research and Review Journal for Microscopy·Berthold HuppertzMaria Kokozidou

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.