PMID: 6406231Jun 15, 1983Paper

Interaction of the dissociable glycerol-3-phosphate dehydrogenase and fructose-1,6-bisphosphate aldolase. Quantitative analysis by an extrinsic fluorescence probe

European Journal of Biochemistry
J OvádiJ Batke

Abstract

Cytoplasmic sn-glycerol-3-phosphate dehydrogenase, labelled covalently with fluorescein isothiocyanate, shows an enzyme-concentration-dependent fluorescence anisotropy. The anisotropy versus enzyme concentration curve is shifted towards higher concentrations when substrates are present. The comparison of the dissociation constants estimated from anisotropy measurements and derived from kinetic experiments suggests that the substrate-induced dissociation of the dimeric dehydrogenase is slow with respect to the enzymatic reaction catalyzed by either its monomeric or dimeric form. The fluorescence anisotropy of the fluorescent dye-labelled dehydrogenase increase with time upon addition of unlabelled fructose-1,6-bisphosphate aldolase approaching a limiting value. This fact indicates the binding of fructose-1,6-bisphosphate aldolase aldose aldolase to glycerolphosphate dehydrogenase. A model is proposed assuming simultaneous binding of tetrameric fructose-1,6-bisphosphate aldolase to monomeric and dimeric glycerolphosphate dehydrogenase with 1:1 stoichiometry. The dissociation constants, as parameters fitted to the experimental curves, were estimated as 0.2 microM and 1 microM for aldolase-dimeric-glycerolphosphate-dehydrogenase an...Continue Reading

References

Aug 16, 1976·European Journal of Biochemistry·M EngelhardR Jaenicke
Jan 1, 1976·Advances in Enzyme Regulation·T KeletiF Bartha
Apr 1, 1978·European Journal of Biochemistry·J Ovádi, T Keleti
May 25, 1971·Biochimica Et Biophysica Acta·A Pesce, G C Schito
Feb 1, 1971·The Biochemical Journal·C J Masters, D J Winzon
Nov 17, 1967·Science·P A Srere
Sep 29, 1981·Biochemistry·L P WakelinM J Waring
Jun 4, 1982·Biochimica Et Biophysica Acta·M Kálmán, L Boross

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Citations

Jan 1, 1989·Progress in Biophysics and Molecular Biology·T KeletiJ Batke
Mar 3, 1986·European Journal of Biochemistry·J KovárW Maret
Apr 6, 2001·Metabolic Engineering·W van WindenS Heijnen
Sep 30, 1987·Biochemical and Biophysical Research Communications·F OroszJ Ovádi
Oct 21, 1985·Journal of Theoretical Biology·B I KurganovL S Mil'man
Nov 5, 2021·Protein Science : a Publication of the Protein Society·Kathryn D FentonAudrey L Lamb

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