Interaction of the epidermal growth factor receptor and the DNA-dependent protein kinase pathway following gefitinib treatment

Molecular Cancer Therapeutics
Benjamin FriedmannDaniel Hochhauser

Abstract

The epidermal growth factor receptor (EGFR) is an important target for cancer therapy. We previously showed that the EGFR inhibitor gefitinib modulated repair of DNA damage following exposure to cisplatin and etoposide involving the DNA-dependent protein kinase (DNA-PK) pathway. In this study, we specifically investigated the effect of EGFR inhibition by gefitinib on functional activity of DNA-PK in cancer cell lines and the interaction between EGFR and DNA-PK. The effects of DNA-PK inhibition by wortmannin and small interfering RNA to the catalytic subunit of DNA-PK (DNA-PK(CS)) on cell proliferation and DNA interstrand cross-link repair were investigated in the human MCF-7 breast cancer cell line and compared with the effects of gefitinib. DNA-PK activity was quantitated and expression measured by immunoblotting following gefitinib treatment. Immunoprecipitation experiments were done with and without gefitinib in MCF-7 cells, the AR42J pancreas cell line with high EGFR, and the human MDA-453 breast cancer cell line expressing low EGFR. Nuclear and cytoplasmic extracts were immunoblotted with antibody to DNA-PK(CS) to determine if gefitinib treatment altered cellular expression. Reduction of DNA-PK activity by wortmannin and e...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·J S Lai, W Herr
Jul 4, 1990·Journal of the National Cancer Institute·P SkehanM R Boyd
Jul 1, 1995·Critical Reviews in Oncology/hematology·D S SalomonN Normanno
Sep 25, 1998·The Journal of Biological Chemistry·S KumarS Kharbanda
Nov 12, 1998·Trends in Biochemical Sciences·S E Critchlow, S P Jackson
May 18, 2001·Nature·J H Hoeijmakers
Mar 23, 2002·Science·Thijn R BrummelkampReuven Agami
Feb 18, 2004·International Journal of Radiation Oncology, Biology, Physics·Paul M Harari, Shyh-Min Huang
Mar 3, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Giuseppe GiacconeDavid H Johnson
Mar 3, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Roy S HerbstDavid H Johnson
Jul 21, 2004·The Journal of Biological Chemistry·Jianhua FengBrian A Hemmings
Jul 23, 2004·The New England Journal of Medicine·David CunninghamEric Van Cutsem
Jul 31, 2004·Science·Raffaella SordellaJeffrey Settleman
Aug 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·William PaoHarold Varmus
Oct 12, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Benjamin FriedmannDaniel Hochhauser
Nov 17, 2004·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Timothy Whelan, Mark Levine
Jan 29, 2005·The EMBO Journal·Ana-Maria DragoiWen-Ming Chu
Mar 10, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·David B SolitFrank M Sirotnak
Apr 14, 2005·Molecular Cancer Therapeutics·David McKillopTrevor C Stephens

❮ Previous
Next ❯

Citations

Apr 2, 2008·Current Oncology Reports·Ranee MehraPaul M Harari
Jul 3, 2013·The Lancet Oncology·Barbara BurtnessThomas Galloway
Apr 3, 2009·Chemical Reviews·Philip J Tofilon, Kevin Camphausen
Oct 13, 2006·Nature Reviews. Cancer·Mukesh K NyatiTheodore S Lawrence
Feb 19, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Toshimitsu TanakaRaymond E Meyn
Nov 5, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Meredith A MorganTheodore S Lawrence
Apr 12, 2014·Nature Reviews. Cancer·Robert C Baxter
Oct 10, 2008·The British Journal of Radiology·C M WestS Bhana
Nov 10, 2009·International Journal of Radiation Biology·Mechthild KrauseMichael Baumann
Dec 7, 2007·International Journal of Radiation Biology·H Peter RodemannMahmoud Toulany
Feb 17, 2009·Cancer Investigation·Jianjian DaiChunyan Ji
Jan 25, 2011·Journal of Thoracic Oncology : Official Publication of the International Association for the Study of Lung Cancer·Chun-Ming TsaiKuo-Ting Chang
Jul 21, 2009·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Raymond E MeynK Kian Ang
Jan 23, 2009·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Jen-Chung KoYun-Wei Lin
Feb 2, 2008·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Klaus DittmannH Peter Rodemann
Nov 27, 2007·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Gabriele WannerKlaus Dittmann
Aug 24, 2007·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Klaus DittmannH Peter Rodemann
May 16, 2007·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Michael BaumannH Peter Rodemann
Mar 21, 2007·Cancer Letters·Pinaki R Dutta, Amit Maity
Jul 25, 2015·Pharmacology & Therapeutics·Kyle C CuneoTheodore S Lawrence
Jun 24, 2014·The International Journal of Biochemistry & Cell Biology·Elzbieta PastwaRichard I Somiari
Sep 21, 2011·Seminars in Cell & Developmental Biology·Ian G Mills
Jul 5, 2011·Radiotherapy and Oncology : Journal of the European Society for Therapeutic Radiology and Oncology·Mahmoud ToulanyH Peter Rodemann
Feb 9, 2012·Cancer Biology & Therapy·Tsuyoshi OhtaHirohisa Kurachi
Oct 12, 2010·Acta Biochimica Et Biophysica Sinica·Yuan YaoZhijun Xi
Feb 28, 2019·Journal of Cellular Physiology·Setareh RezatabarBahman Yousefi
Dec 21, 2019·International Journal of Radiation Biology·Siyuan TangYa Wang
Apr 6, 2013·The Journal of Clinical Endocrinology and Metabolism·Francesco FrascaRiccardo Vigneri
Feb 7, 2007·The Journal of Biological Chemistry·Sheng-Chieh Hsu, Mien-Chie Hung
Sep 2, 2018·The Journal of Biological Chemistry·Shiuh-Rong Ho, Weei-Chin Lin
Jan 11, 2019·Journal of Experimental & Clinical Cancer Research : CR·Zhangjian ZhouDunfa Peng

❮ Previous
Next ❯

Related Concepts

Related Papers

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Benjamin FriedmannDaniel Hochhauser
The New England Journal of Medicine
Steven M Sorscher
The New England Journal of Medicine
Giulio RossiLucia Longo
The New England Journal of Medicine
Jeremy N RichHai Yan
© 2021 Meta ULC. All rights reserved