Interaction of the erythropoietin and stem-cell-factor receptors

Nature
H WuH F Lodish

Abstract

Mutations in the KIT transmembrane protein-tyrosine kinase receptor affect erythropoiesis, resulting in fewer committed late progenitors (colony-forming unit erythroid, CFU-E) in the fetal liver. As the survival and proliferation of CFU-Es depend absolutely on erythropoietin (EPO), these results suggest that CFU-Es cannot proliferate or mature further unless both the KIT and EPO receptor signalling pathways are functional. How KIT affects proliferation or differentiation of CFU-Es is not clear. Here we show that the KIT ligand SCF (for stem-cell factor) can replace EPO in supporting the growth and survival of HCD57 cells, an EPO-dependent erythroid-progenitor cell line expressing high levels of KIT. SCF supports the proliferation of 32D cells that express KIT only if they also express the EPO receptor. In HCD57 cells, SCF rapidly induces tyrosine phosphorylation of the EPO receptor, and KIT physically associates with the extended box 2 region in the cytoplasmic domain of the EPO receptor. Our results indicate that KIT may activate the EPO receptor by tyrosine phosphorylation to induce further proliferation and maturation of CFU-Es.

References

Feb 1, 1992·Molecular and Cellular Biology·A Yoshimura, H F Lodish
Jun 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·A YoshimuraH F Lodish
Nov 1, 1988·Molecular and Cellular Biology·S MajumderP Besmer
Apr 21, 1989·Cell·A D D'AndreaG G Wong
Jan 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·D J HiltonH F Lodish
Jul 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S T Sawyer, W D Hankins

❮ Previous
Next ❯

Citations

Jan 1, 1996·Developmental Genetics·L A MegeneyM A Rudnicki
May 20, 1998·Biotherapy·A R Migliaccio, G Migliaccio
Dec 15, 2011·Endocrine·Cristina Hernández, Rafael Simó
Jul 5, 2001·Experimental Hematology·T EndoK Sawada
Aug 10, 2001·Cellular Signalling·T ZhuP E Lobie
May 14, 1999·Trends in Endocrinology and Metabolism : TEM·S N ConstantinescuH F Lodish
Dec 3, 1999·The International Journal of Biochemistry & Cell Biology·P A Tilbrook, S P Klinken
Dec 3, 1999·The International Journal of Biochemistry & Cell Biology·D Linnekin
Nov 10, 1998·Nature Structural Biology·M D Ballinger, J A Wells
May 2, 2001·Nature Reviews. Molecular Cell Biology·T Taniguchi, A Takaoka
Jul 10, 2013·Integrative Biology : Quantitative Biosciences From Nano to Macro·Amir ShamlooSarah Heilshorn
Jul 23, 1998·British Journal of Haematology·H KurataG Migliaccio
Sep 30, 1998·British Journal of Haematology·T KitohS Nakagawa
Oct 16, 1999·British Journal of Haematology·J M FreyssinierS Fichelson
Jul 28, 2001·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·Y MitaniT Taniguchi
Mar 9, 2011·Proceedings of the National Academy of Sciences of the United States of America·Zhi-jia YeSherman M Weissman
Apr 2, 1996·Proceedings of the National Academy of Sciences of the United States of America·P O IversenA F Lopez
Oct 24, 2001·Journal of Hematotherapy & Stem Cell Research·A OdaK Sawada
Apr 7, 2007·Current Opinion in Hematology·Merav Socolovsky
Dec 5, 2009·Artificial Organs·Ahmet A KiykimIbrahim Haznedaroglu
Jun 30, 1998·APMIS. Supplementum·J Olweus
Oct 25, 2006·Molecular and Cellular Biology·Zhifu XiangMichael H Tomasson
Jan 1, 1996·Annual Review of Cell and Developmental Biology·S S WatowichH F Lodish
Jan 1, 1997·Annual Review of Genetics·S H Orkin, L I Zon
Mar 17, 2007·Circulation Research·Paolo Madeddu, Costanza Emanueli
Mar 3, 2006·The Journal of Clinical Investigation·Madhu P MenonDon M Wojchowski
Jul 21, 2000·The Journal of Clinical Investigation·T SatoT Nakahata
Dec 8, 2005·Blood·Madhu P MenonDon M Wojchowski
Jun 8, 2007·Blood·Thomas JahnKenneth Weinberg
Feb 2, 2008·Blood·Florian GrebienRichard Moriggl

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Cell Signaling by Tyrosine Kinases

Receptor tyrosine kinases (RTKs) are the high-affinity cell surface receptors for many polypeptide growth factors, cytokines, and hormones. RTKs have been shown not only to be key regulators of normal cellular processes but also to have a critical role in the development and progression of many types of cancer. Discover the latest research on cell signaling and RTK here.