Interaction of the exported malaria protein Pf332 with the red blood cell membrane skeleton.

Biochimica Et Biophysica Acta
Karena L WallerBrian M Cooke

Abstract

Intra-erythrocytic Plasmodium falciparum malaria parasites synthesize and export numerous proteins into the red blood cell (RBC) cytosol, where some bind to the RBC membrane skeleton. These interactions are responsible for the altered antigenic, morphological and functional properties of parasite-infected red blood cells (IRBCs). Plasmodium falciparum protein 332 (Pf332) is a large parasite protein that associates with the membrane skeleton and who's function has recently been elucidated. Using recombinant fragments of Pf332 in in vitro interaction assays, we have localised the specific domain within Pf332 that binds to the RBC membrane skeleton to an 86 residue sequence proximal to the C-terminus of Pf332. We have shown that this region partakes in a specific and saturable interaction with actin (K(d)=0.60 microM) but has no detectable affinity for spectrin. The only exported malaria protein previously known to bind to actin is PfEMP3 but here we demonstrate that there is no competition for actin-binding between PfEMP3 and Pf332, suggesting that they bind to different target sequences in actin.

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Citations

Apr 11, 2013·Proceedings of the National Academy of Sciences of the United States of America·Hiromi IkadaiMarcelo Jacobs-Lorena
Sep 13, 2011·Eukaryotic Cell·Geoffrey K Kilili, Douglas J LaCount
Jul 25, 2013·Blood Cells, Molecules & Diseases·Surendra K Prajapati, Om P Singh
Sep 18, 2012·Trends in Parasitology·Marek CyrklaffMichael Lanzer
Oct 10, 2012·Molecular and Biochemical Parasitology·Marcel DeponteJude M Przyborski
Feb 9, 2012·Malaria Research and Treatment·Sandra NilssonQijun Chen
Mar 18, 2011·Plant, Cell & Environment·Renate Scheibe, Karl-Josef Dietz
Jul 5, 2016·Nature Reviews. Microbiology·Tania F de Koning-WardPaul R Gilson
Sep 6, 2019·Microbiology and Molecular Biology Reviews : MMBR·Jan D Warncke, Hans-Peter Beck
Sep 3, 2016·FEMS Microbiology Reviews·Emma S Sherling, Christiaan van Ooij
Jun 20, 2017·Molecular and Biochemical Parasitology·Bikash ShakyaDouglas J LaCount
Apr 13, 2021·Frontiers in Cellular and Infection Microbiology·Margaux ChauvetAnaïs Merckx

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