PMID: 6538483Apr 2, 1984Paper

Interaction of the HB protein of Bacillus globigii with nucleic acids. Analysis of the binding to DNA and polynucleotides

European Journal of Biochemistry
F WatanabeG Schwarz

Abstract

Fluorescence of the phenylalanine residues of the HB protein of Baccillus globigii was found to be quenched upon binding to nucleic acids. Using this phenomenon, the binding properties were investigated on the basis of an approach published recently. The stoichiometric numbers were about 10 basepairs per bound protein molecule for double-stranded DNA and about 10 bases for single-stranded polynucleotides, independent of salt concentration. Cooperativity parameters were in the range of 50-250. Binding constants were about 5 X 10(6) M-1 (at 0.1 M NaCl) and decreased with increasing salt concentration. From the salt dependence it is inferred that about one Na+ ion is displaced upon binding of a protein molecule to DNA. Therefore the binding site should contain one positively charged amino acid residue. The protein was found to bind with comparable strength to double-stranded and single-stranded DNA as well as to poly(rA). Hence it is concluded that the HB protein does not belong to the category of 'melting proteins'.

References

Jul 23, 1975·Biochimica Et Biophysica Acta·D G Searcy
Jun 1, 1976·Proceedings of the National Academy of Sciences of the United States of America·R Haselkorn, J Rouvière-Yaniv
Nov 4, 1976·Journal of Molecular Biology·M T RecordP De Haseth
Sep 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J Rouvière-Yaniv, F Gros
Dec 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·R Cukier-KahnF Gros

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Citations

Apr 25, 1986·Nucleic Acids Research·F Watanabe
Nov 11, 1994·Nucleic Acids Research·M L Nedved, G R Moe
Jun 15, 1987·European Journal of Biochemistry·R ImberU Heinemann
Jun 23, 2012·Molecular Biology Reports·Tangirala SuryanarayanaUsha Kumari Garapati
Apr 23, 2005·The Biochemical Journal·Edwin KamauAnne Grove
Jan 1, 1985·Bio Systems·P J Rizzo

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