Interaction of the Hsp110 molecular chaperones from S. cerevisiae with substrate protein

Journal of Molecular Biology
Sigrun PolierAndreas Bracher

Abstract

Hsp110 proteins act as nucleotide exchange factors of the molecular chaperone Hsp70 in eukaryotes. In addition, they have been reported to stabilize unfolded proteins for subsequent refolding. Hsp110 proteins belong to the Hsp70 superfamily and, in analogy to Hsp70, the substrate-binding site was proposed to be located at the interface of the beta-sandwich domain and the three-helix-bundle domain. Saccharomyces cerevisiae has two closely related cytosolic isoforms of Hsp110, Sse1p and Sse2p. Under normal growth conditions, Sse1p is the predominant form. Sse2p is induced under stress conditions, such as heat shock. Consistent with these findings, we find that Sse2p has increased temperature stability. Both Sse1p and Sse2p accelerate nucleotide exchange on the yeast Hsp70 Ssa1p. Furthermore, Sse1p and Sse2p effectively compete for binding of unfolded luciferase. In contrast to Sse1p, however, Sse2p fails to stabilize this model substrate under thermal stress for subsequent Hsp70-mediated refolding. Using a domain shuffling approach, we show that both the nucleotide-binding domain and the beta-sandwich domain of Sse1p are required to preserve nonnative luciferase in a folding-competent state. Our findings suggest that Sse1p must u...Continue Reading

References

Apr 7, 1997·Biochemical and Biophysical Research Communications·Y KanekoJ Fujita
May 21, 1999·The Journal of Biological Chemistry·H J OhJ R Subjeck
Oct 26, 2000·Cell Stress & Chaperones·D P EastonJ R Subjeck
Mar 9, 2002·Science·F Ulrich Hartl, Manajit Hayer-Hartl
Aug 16, 2002·Molecular Biology of the Cell·Jennifer L GoeckelerJeffrey L Brodsky
Oct 17, 2003·Nature·Sina GhaemmaghamiJonathan S Weissman
Mar 17, 2005·Cellular and Molecular Life Sciences : CMLS·M P Mayer, B Bukau
May 7, 2005·Journal of Molecular Biology·Matthew RevingtonErik R P Zuiderweg
Oct 6, 2005·Nature Structural & Molecular Biology·Martin HaslbeckJohannes Buchner
Nov 26, 2005·Molecular Cell·Jianwen JiangRui Sousa
Dec 21, 2005·FEBS Letters·Holger RaviolMatthias P Mayer
Apr 15, 2006·The Journal of Biological Chemistry·Wolfgang RistMatthias P Mayer
May 12, 2006·The EMBO Journal·Zdravko DragovicF Ulrich Hartl
Jul 24, 2007·Experimental Cell Research·Youhei SaitoTakumi Hatayama
Jun 10, 2008·FEBS Letters·Jennifer L GoeckelerJeffrey L Brodsky
Jun 14, 2008·Molecular Cell·Jonathan P SchuermannRui Sousa
Jan 28, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jiou WangArthur L Horwich
Jun 6, 2009·Nature Structural & Molecular Biology·F Ulrich Hartl, Manajit Hayer-Hartl

❮ Previous
Next ❯

Citations

May 3, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jennifer R MorganEileen M Lafer
Jan 15, 2013·Genetics and Molecular Research : GMR·Z J LiuJ Hartung
Oct 27, 2015·Frontiers in Molecular Biosciences·Nadinath B Nillegoda, Bernd Bukau
Sep 20, 2012·The EMBO Journal·Heike RampeltBernd Bukau
May 31, 2011·Veterinary Parasitology·Zhancheng TianJin Luo
Feb 26, 2016·Frontiers in Molecular Biosciences·Andreas Bracher, Jacob Verghese
Nov 19, 2014·PLoS Biology·Daniela Martino RothWilliam E Balch
Nov 21, 2018·The Journal of Biological Chemistry·Matthias P Mayer, Lila M Gierasch
Dec 12, 2013·The Journal of Biological Chemistry·Julia Behnke, Linda M Hendershot
Nov 30, 2019·International Journal of Molecular Sciences·Tawanda Zininga, Addmore Shonhai
Mar 29, 2014·The Journal of Biological Chemistry·Jennifer L AbramsKevin A Morano
Jul 8, 2019·Biophysics Reviews·Graham ChakafanaAddmore Shonhai
Mar 24, 2021·The Journal of Biological Chemistry·Unekwu M Yakubu, Kevin A Morano

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Proceedings of the National Academy of Sciences of the United States of America
Claes AndréassonBernd Bukau
The Korean Journal of Physiology & Pharmacology : Official Journal of the Korean Physiological Society and the Korean Society of Pharmacology
Hyun Jeong KimJunesun Kim
© 2021 Meta ULC. All rights reserved