Interaction of the insulin receptor kinase with serine/threonine kinases in vitro

Journal of Cellular Biochemistry
H HäringP J Roach

Abstract

Insulin causes rapid phosphorylation of the beta subunit (Mr = 95,000) of its receptor in broken cell preparations. This occurs on tyrosine residues and is due to activation of a protein kinase which is contained in the receptor itself. In the intact cell, insulin also stimulates the phosphorylation of the receptor and other cellular proteins on serine and threonine residues. In an attempt to find a protein that might link the receptor tyrosine kinase to these serine/threonine phosphorylation reactions, we have studied the interaction of a partially purified preparation of insulin receptor with purified preparations of serine/threonine kinases known to phosphorylate glycogen synthase. No insulin-dependent phosphorylation was observed when casein kinases I and II, phosphorylase kinase, or glycogen synthase kinase 3 was incubated in vitro with the insulin receptor. These kinases also failed to phosphorylate the receptor. By contrast, the insulin receptor kinase catalyzed the phosphorylation of the calmodulin-dependent kinase and addition of insulin in vitro resulted in a 40% increase in this phosphorylation. In the presence of calmodulin-dependent kinase and the insulin receptor kinase, insulin also stimulated the phosphorylation...Continue Reading

References

Jan 1, 1981·Current Topics in Cellular Regulation·P J Roach
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May 1, 1983·Diabetes·D E McMillan
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Citations

Jan 1, 1988·The International Journal of Biochemistry·G J Sale
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·H HäringB Obermaier
Feb 1, 1991·Clinical Endocrinology·R Taylor
Jan 1, 1986·Annals of the New York Academy of Sciences·J M McDonaldJ Colca
May 4, 2005·Protein Expression and Purification·Valentina SalasAntonio Villalobo
Aug 1, 1989·Diabetes/metabolism Reviews·H Häring, B Obermaier-Kusser
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Related Concepts

Cyclic AMP-Phosphodiesterase Activator
Histone H7
Phosphorylation
Protein Conformation
Protein KINASE
Protein Tyrosine Kinase
Insulin Receptor beta Subunit
Substrate Specificity
Protein-Serine-Threonine Kinases

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