Interaction of the mediator head module with RNA polymerase II.

Structure
Gang CaiFrancisco J Asturias

Abstract

Mediator, a large (21 polypeptides, MW ∼1 MDa) complex conserved throughout eukaryotes, plays an essential role in control of gene expression by conveying regulatory signals that influence the activity of the preinitiation complex. However, the precise mode of interaction between Mediator and RNA polymerase II (RNAPII), and the mechanism of regulation by Mediator remain elusive. We used cryo-electron microscopy and reconstituted in vitro transcription assays to characterize a transcriptionally-active complex including the Mediator Head module and components of a minimum preinitiation complex (RNAPII, TFIIF, TFIIB, TBP, and promoter DNA). Our results reveal how the Head interacts with RNAPII, affecting its conformation and function.

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Citations

Nov 11, 2014·Nature Structural & Molecular Biology·Murat A CevherRobert G Roeder
May 9, 2014·Cell Research·Xuejuan WangGang Cai
Mar 12, 2013·The Journal of Biological Chemistry·Banupriya Mukundan, Athar Ansari
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Apr 9, 2013·Nature Structural & Molecular Biology·Kuang-Lei TsaiFrancisco J Asturias
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Aug 22, 2020·Annual Review of Cell and Developmental Biology·Sara Osman, Patrick Cramer
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